The glutamyl tail length of the cofactor F 420 in the methanogenic Archaea Methanosarcina thermophila and Methanoculleus thermophilus.

Autor: Wunderer M; Universität Innsbruck, Department of Microbiology, Technikerstraße 25d, 6020 Innsbruck, Austria. Electronic address: Mathias.Wunderer@uibk.ac.at., Markt R; Universität Innsbruck, Department of Microbiology, Technikerstraße 25d, 6020 Innsbruck, Austria., Lackner N; Universität Innsbruck, Department of Microbiology, Technikerstraße 25d, 6020 Innsbruck, Austria., Wagner AO; Universität Innsbruck, Department of Microbiology, Technikerstraße 25d, 6020 Innsbruck, Austria.
Jazyk: angličtina
Zdroj: The Science of the total environment [Sci Total Environ] 2022 Feb 25; Vol. 809, pp. 151112. Date of Electronic Publication: 2021 Oct 21.
DOI: 10.1016/j.scitotenv.2021.151112
Abstrakt: The cofactor F 420 is synthesized by many different organisms and as a redox cofactor, it plays a crucial role in the redox reactions of catabolic and biosynthetic metabolic pathways. It consists of a deazaflavin structure, which is linked via lactate to an oligoglutamate chain, that can vary in length. In the present study, the methanogenic Archaea Methanosarcina thermophila and Methanoculleus thermophilus were cultivated on different carbon sources and their coenzyme F 420 composition has been assayed by reversed-phase ion-pair high-performance liquid chromatography with fluorometric detection regarding both, overall cofactor F 420 production and distribution of F 420 glutamyl tail length. In Methanosarcina thermophila cultivated on methanol, acetate, and a mixture of acetate and methanol, the most abundant cofactors were F 420 -5 and F 420 -4, whereby the last digit refers to the number of expressed glutamyl rests. By contrast, in the obligate CO 2 reducing Methanoculleus thermophilus the most abundant cofactors were F 420 -3 and F 420 -4. In Methanosarcina thermophila, the relative proportions of the expressed F 420 tail length changed during batch growth on all three carbon sources. Over time F 420 -3 and F 420 -4 decreased while F 420 -5 and F 420 -6 increased in their relative proportion in comparison to total F 420 content. In contrast, in Methanoculleus thermophilus the relative abundance of the different F 420 cofactors remained stable. It was also possible to differentiate the two methanogenic Archaea based on the glutamyl tail length of the cofactor F 420 . The cofactor F 420 -5 in concentrations >2% could only be assigned to Methanosarcina thermophila. In all four variants a trend for a positive correlation between the DNA concentration and the total concentration of the cofactor could be shown. Except for the variant Methanosarcinathermophila with acetate as sole carbon source the same could be shown between the concentration of the mcrA gene copy number and the total concentration of the cofactor.
Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2021 The Authors. Published by Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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