Comparative study on the effect of aspirin, TP receptor antagonist and TxA 2 synthase inhibitor on the vascular tone of human saphenous vein and internal mammary artery.

Autor: Ozen G; Department of Pharmacology, Faculty of Pharmacy, Istanbul University, Istanbul, Turkey. Electronic address: gulsevozen@istanbul.edu.tr., Aljesri K; Department of Pharmacology, Faculty of Pharmacy, Istanbul University, Istanbul, Turkey., Abdelazeem H; Université de Paris, INSERM, UMR-S 1148, CHU X. Bichat, 75018 Paris, France; Université Sorbonne Paris Nord, 93430 Villetaneuse, France; Department of Pharmacology and Toxicology, Faculty of Pharmacy, Alexandria University, Egypt., Norel X; Université de Paris, INSERM, UMR-S 1148, CHU X. Bichat, 75018 Paris, France; Université Sorbonne Paris Nord, 93430 Villetaneuse, France., Turkyılmaz G; Department of Cardiovascular Surgery, Bakirkoy Dr Sadi Konuk Education and Research Hospital Bakırkoy, Istanbul, Turkey., Turkyılmaz S; Department of Cardiovascular Surgery, Bakirkoy Dr Sadi Konuk Education and Research Hospital Bakırkoy, Istanbul, Turkey., Topal G; Department of Pharmacology, Faculty of Pharmacy, Istanbul University, Istanbul, Turkey.
Jazyk: angličtina
Zdroj: Life sciences [Life Sci] 2021 Dec 01; Vol. 286, pp. 120073. Date of Electronic Publication: 2021 Oct 21.
DOI: 10.1016/j.lfs.2021.120073
Abstrakt: Aims: Thromboxane (TxA 2 ) is synthesized from arachidonic acid (AA) via thromboxane synthase (TxS) enzyme and induces vasoconstriction via TP receptor. Our aim is to compare the effects of aspirin, TxS inhibitor and TP receptor antagonist on vascular reactivity of bypass grafts (saphenous vein and internal mammary artery).
Main Methods: Using isolated organ bath, saphenous vein and internal mammary artery preparations were incubated with TP receptor antagonist, TxS inhibitor, aspirin, IP or EP4 receptor antagonist. Then prostaglandin (PG)E 2 , PGF 2α , phenylephrine and AA were administered in concentration-dependent manner. The expression of prostanoid receptor and PGI 2 synthase (PGIS) enzyme was determined by Western Blot.
Key Findings: TP receptor antagonist inhibited the contraction induced by PGE 2 , PGF 2α , and AA but not that induced by phenylephrine in both types of vessels. Aspirin increased phenylephrine-induced contraction only in internal mammary artery and decreased AA-induced contraction in saphenous vein. TxS inhibitor decreased both PGE 2 and AA-induced contraction in both types of vessels. This decrease was reversed by co-incubation of TxS inhibitor and IP/EP4 receptor antagonists. The expressions of EP3 receptor and PGIS enzyme were greater in internal mammary artery compared to saphenous vein while IP and TP receptors expressed at similar levels.
Significance: TP receptor antagonist and TxS inhibitor are more effective to reduce contraction induced by different spasmogens in comparison to aspirin. Our results suggest that TP receptor antagonist and TxS inhibitor might have an advantage over aspirin due to their preventive effect on increased vascular reactivity observed in post-operative period of coronary artery bypass grafting.
(Copyright © 2021 Elsevier Inc. All rights reserved.)
Databáze: MEDLINE
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