Isolation of bioactive compounds from lichen Parmelia sulcata and evaluation of antimicrobial property.

Autor:	Gandhi AD; Department of Biotechnology, Thiruvalluvar University, Serkkadu, Vellore-632115, Tamil Nadu, India; Department of Chemical Technology, Faculty of Science, Chulalongkorn University, Bangkok, Thailand. Electronic address: dhaneshmsc@gmail.com., Umamahesh K; Department of Biochemistry, Sri Venkateswara University, Tirupati 517502, Andhra Pradesh, India., Sathiyaraj S; Department of Biotechnology, Thiruvalluvar University, Serkkadu, Vellore-632115, Tamil Nadu, India., Suriyakala G; Department of Biotechnology, Thiruvalluvar University, Serkkadu, Vellore-632115, Tamil Nadu, India., Velmurugan R; Faculty of Science, Department of Biochemistry, Chulalongkorn University, Bangkok, Thailand., Al Farraj DA; Department of Botany and Microbiology, College of Sciences, King Saud University, P.O. Box 22452, Riyadh 11495, Saudi Arabia., Gawwad MRA; Genetics & Bioengineering, Faculty of Engineering and Natural Sciences, International University of Sarajevo, Bosnia and Herzegovina., Murugan K; Department of Zoology, Bharathiar University, Coimabatore, India., Babujanarthanam R; Department of Biotechnology, Thiruvalluvar University, Serkkadu, Vellore-632115, Tamil Nadu, India. Electronic address: babukmg@gmail.com., Saranya R; Department of Microbiology, Sacred Heart College, Tirupattur, India.
Jazyk:	angličtina
Zdroj:	Journal of infection and public health [J Infect Public Health] 2022 Apr; Vol. 15 (4), pp. 491-497. Date of Electronic Publication: 2021 Oct 14.
DOI:	10.1016/j.jiph.2021.10.014
Abstrakt:	Background: Lichens were used as an ailment in the traditional medicine for treating various disorders for centuries. Since there is less evidence in the literature about the medicinal property of Parmelia sulcata (P. sulcata), we made a pioneer attempt to explore the antioxidant and antimicrobial properties of lichens. Methods: In the present study, the three Samples were collected by using the column chromatography by elucidating the ethyl acetate extract of P. sulcata, and the samples were subjected to DPPH and ABTS assays to find the free radical scavenging activity, total phenols and flavonoids were estimated. The minimum inhibitory concentration was evaluated against the bacterial species (Bacillus subtilis, Pseudomonas aeruginosa, Escherichia coli and Klebsiella pneumoniae) and fungal species (Candida albicans and Aspergillus fumigatus) by the microdilution method. The best activity sample was analyzed using the Gas Chromatography-Mass Spectrometry (GC-MS), Fourier Transmission Infrared Spectroscopy (FT-IR) and Nuclear Magnetic Resonance (NMR). Results: The results shown that all the samples contain phenols and flavonoids which are responsible for antioxidants, antibacterial and antifungal activities. Among that sample-3 shown best antimicrobial activity and it was analyzed and identified as 7-hydroxy-3-(2-methylbut-3-en2-yl)-chromen-2-one. Conclusion: The outcome of the study suggests that sample-3 shown good antimicrobial activity and identified as 7-hydroxy-3-(2-methylbut-3-en2-yl)-chromen-2-one. It can be a resource for further studies. (Copyright © 2021 The Author(s). Published by Elsevier Ltd.. All rights reserved.)
Databáze:	MEDLINE
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