Turnover of fear engram cells by repeated experience.

Autor:	Cho HY; Department of Biological Sciences, Korea Advanced Institute of Science and Technology (KAIST), Yuseong-gu, Daejeon 34141, Korea; KAIST Institute for the BioCentury (KIB), Korea Advanced Institute of Science and Technology (KAIST), Yuseong-gu, Daejeon 34141, Korea., Shin W; Center for Synaptic Brain Dysfunctions, Institute for Basic Science (IBS), Daejeon 34141, Korea., Lee HS; Department of Biological Sciences, Korea Advanced Institute of Science and Technology (KAIST), Yuseong-gu, Daejeon 34141, Korea; KAIST Institute for the BioCentury (KIB), Korea Advanced Institute of Science and Technology (KAIST), Yuseong-gu, Daejeon 34141, Korea., Lee Y; Department of Biological Sciences, Korea Advanced Institute of Science and Technology (KAIST), Yuseong-gu, Daejeon 34141, Korea; KAIST Institute for the BioCentury (KIB), Korea Advanced Institute of Science and Technology (KAIST), Yuseong-gu, Daejeon 34141, Korea., Kim M; Department of Biological Sciences, Korea Advanced Institute of Science and Technology (KAIST), Yuseong-gu, Daejeon 34141, Korea; KAIST Institute for the BioCentury (KIB), Korea Advanced Institute of Science and Technology (KAIST), Yuseong-gu, Daejeon 34141, Korea., Oh JP; Department of Biological Sciences, Korea Advanced Institute of Science and Technology (KAIST), Yuseong-gu, Daejeon 34141, Korea; KAIST Institute for the BioCentury (KIB), Korea Advanced Institute of Science and Technology (KAIST), Yuseong-gu, Daejeon 34141, Korea., Han J; Department of Biological Sciences, Korea Advanced Institute of Science and Technology (KAIST), Yuseong-gu, Daejeon 34141, Korea; KAIST Institute for the BioCentury (KIB), Korea Advanced Institute of Science and Technology (KAIST), Yuseong-gu, Daejeon 34141, Korea., Jeong Y; Department of Biological Sciences, Korea Advanced Institute of Science and Technology (KAIST), Yuseong-gu, Daejeon 34141, Korea; KAIST Institute for the BioCentury (KIB), Korea Advanced Institute of Science and Technology (KAIST), Yuseong-gu, Daejeon 34141, Korea., Suh B; Department of Biological Sciences, Korea Advanced Institute of Science and Technology (KAIST), Yuseong-gu, Daejeon 34141, Korea; KAIST Institute for the BioCentury (KIB), Korea Advanced Institute of Science and Technology (KAIST), Yuseong-gu, Daejeon 34141, Korea., Kim E; Department of Biological Sciences, Korea Advanced Institute of Science and Technology (KAIST), Yuseong-gu, Daejeon 34141, Korea; Center for Synaptic Brain Dysfunctions, Institute for Basic Science (IBS), Daejeon 34141, Korea., Han JH; Department of Biological Sciences, Korea Advanced Institute of Science and Technology (KAIST), Yuseong-gu, Daejeon 34141, Korea; KAIST Institute for the BioCentury (KIB), Korea Advanced Institute of Science and Technology (KAIST), Yuseong-gu, Daejeon 34141, Korea. Electronic address: han.jinhee@kaist.ac.kr.
Jazyk:	angličtina
Zdroj:	Current biology : CB [Curr Biol] 2021 Dec 20; Vol. 31 (24), pp. 5450-5461.e4. Date of Electronic Publication: 2021 Oct 22.
DOI:	10.1016/j.cub.2021.10.004
Abstrakt:	A sparse population of neurons active during a learning event has been identified as memory engram cells. However, cells that are recruited to support memory when experience is repeated have been scarcely explored. Evidence from previous studies provides contradictory views. To address these questions, we employed learning-dependent cell labeling in the lateral amygdala (LA) and applied electrophysiological recording, spine imaging, and optogenetic tools to the labeled neurons with or without retraining. We found that engram cells established from original fear learning became dispensable for memory retrieval specifically with relearning, and this correlated with a reduction of synaptic transmission and loss of dendritic spines in these neurons. Despite such decreased connectivity, direct activation of these neurons resulted in fear-memory recall. We further identified that repeated memory was encoded in neurons active during relearning. These results suggest a shift in neuronal ensembles encoding fear memory in the LA by relearning through disconnection of the existing engram neurons established from original experience. Competing Interests: Declaration of interests The authors declare no competing interests. (Copyright © 2021 Elsevier Inc. All rights reserved.)
Databáze:	MEDLINE
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