| Autor: |
García-Sánchez AN; Centro de Investigación en Química Aplicada, Boulevard Enrique Reyna No. 140, Saltillo 25294, Mexico., Yáñez-Macias R; Centro de Investigación en Química Aplicada, Boulevard Enrique Reyna No. 140, Saltillo 25294, Mexico., Hernández-Flores JL; Centro de Investigación y de Estudios Avanzados-Unidad Irapuato, Irapuato 36821, Mexico., Álvarez-Morales A; Centro de Investigación y de Estudios Avanzados-Unidad Irapuato, Irapuato 36821, Mexico., Valenzuela-Soto JH; Centro de Investigación en Química Aplicada, Boulevard Enrique Reyna No. 140, Saltillo 25294, Mexico.; CONACyT-Centro de Investigación en Química Aplicada, Boulevard Enrique Reyna Hermosillo 140, Saltillo 25294, Mexico., Guerrero-Sanchez C; Laboratory of Organic and Macromolecular Chemistry (IOMC), Friedrich Schiller University Jena, Humboldtstr. 10, 07743 Jena, Germany.; Jena Center for Soft Matter (JCSM), Friedrich Schiller University Jena, Philosophenweg 7, 07743 Jena, Germany., Guerrero-Santos R; Centro de Investigación en Química Aplicada, Boulevard Enrique Reyna No. 140, Saltillo 25294, Mexico. |
| Abstrakt: |
Candidatus Liberibacter solanacearum ( Ca Lso) is associated with diseases in tomato crops and transmitted by the tomato psyllid Bactericera cockerelli. A polymeric water-dispersible nanobactericide (PNB) was evaluated against Ca Lso as a different alternative. PNB is a well-defined polycationic diblock copolymer designed to permeate into the vascular system of plants. Its assessment under greenhouse conditions was carried out with tomato plants previously infected with Ca Lso. Using a concentration as low as 1.0 mg L -1 , a small but significant reduction in the bacterial load was observed by real-time qPCR. Thus, to achieve an ecologically friendly dosage and set an optimum treatment protocol, we performed experiments to determine the effective concentration of PNB to reduce ~65% of the initial bacterial load. In a first bioassay, a 40- or 70-fold increase was used to reach that objective. At this concentration level, other bioassays were explored to determine the effect as a function of time. Surprisingly, a real reduction in the symptoms was observed after three weeks, and there was a significant decrease in the bacterial load level (~98%) compared to the untreated control plants. During this period, flowering and formation of tomato fruits were observed in plants treated with PNB. |
