| Autor: |
Beekhof GC; Department of Neuroscience, Erasmus Medical Center, 3015 AA Rotterdam, The Netherlands.; Netherlands Institute for Neuroscience, Royal Academy of Arts and Sciences (KNAW), 1105 BA Amsterdam, The Netherlands., Gornati SV; Department of Neuroscience, Erasmus Medical Center, 3015 AA Rotterdam, The Netherlands., Canto CB; Netherlands Institute for Neuroscience, Royal Academy of Arts and Sciences (KNAW), 1105 BA Amsterdam, The Netherlands., Libster AM; Edmond & Lily Safra Center for Brain Sciences (ELSC), Department of Neurobiology, Institute of Life Sciences, The Hebrew University, Jerusalem 91904, Israel., Schonewille M; Department of Neuroscience, Erasmus Medical Center, 3015 AA Rotterdam, The Netherlands., De Zeeuw CI; Department of Neuroscience, Erasmus Medical Center, 3015 AA Rotterdam, The Netherlands.; Netherlands Institute for Neuroscience, Royal Academy of Arts and Sciences (KNAW), 1105 BA Amsterdam, The Netherlands., Hoebeek FE; Department of Neuroscience, Erasmus Medical Center, 3015 AA Rotterdam, The Netherlands.; Department for Developmental Origins of Disease, Wilhelmina Children's Hospital, Brain Center, University Medical Center Utrecht, 3584 EA Utrecht, The Netherlands. |
| Abstrakt: |
Purkinje cells (PCs) in the cerebellar cortex can be divided into at least two main subpopulations: one subpopulation that prominently expresses ZebrinII (Z+), and shows a relatively low simple spike firing rate, and another that hardly expresses ZebrinII (Z-) and shows higher baseline firing rates. Likewise, the complex spike responses of PCs, which are evoked by climbing fiber inputs and thus reflect the activity of the inferior olive (IO), show the same dichotomy. However, it is not known whether the target neurons of PCs in the cerebellar nuclei (CN) maintain this bimodal distribution. Electrophysiological recordings in awake adult mice show that the rate of action potential firing of CN neurons that receive input from Z+ PCs was consistently lower than that of CN neurons innervated by Z- PCs. Similar in vivo recordings in juvenile and adolescent mice indicated that the firing frequency of CN neurons correlates to the ZebrinII identity of the PC afferents in adult, but not postnatal stages. Finally, the spontaneous action potential firing pattern of adult CN neurons recorded in vitro revealed no significant differences in intrinsic pacemaking activity between ZebrinII identities. Our findings indicate that all three main components of the olivocerebellar loop, i.e., PCs, IO neurons and CN neurons, operate at a higher rate in the Z- modules. |
