| Autor: |
Vashukova ES; Department of Genomic Medicine, D.O. Ott Research Institute for Obstetrics, Gynecology and Reproduction, 199034 St. Petersburg, Russia., Kozyulina PY; Department of Genomic Medicine, D.O. Ott Research Institute for Obstetrics, Gynecology and Reproduction, 199034 St. Petersburg, Russia.; Signal Regulation Laboratory, All-Russia Research Institute for Agricultural Microbiology, 196608 St. Petersburg, Russia., Illarionov RA; Department of Genomic Medicine, D.O. Ott Research Institute for Obstetrics, Gynecology and Reproduction, 199034 St. Petersburg, Russia.; Institute of Translational Biomedicine, St. Petersburg State University, 199034 St. Petersburg, Russia.; Department of Chemical and Biotechnology, St. Petersburg State Institute of Technology, Technical University, 190013 St. Petersburg, Russia., Yurkina NO; Department of Genomic Medicine, D.O. Ott Research Institute for Obstetrics, Gynecology and Reproduction, 199034 St. Petersburg, Russia., Pachuliia OV; Department of Genomic Medicine, D.O. Ott Research Institute for Obstetrics, Gynecology and Reproduction, 199034 St. Petersburg, Russia., Butenko MG; Department of Genomic Medicine, D.O. Ott Research Institute for Obstetrics, Gynecology and Reproduction, 199034 St. Petersburg, Russia., Postnikova TB; Department of Genomic Medicine, D.O. Ott Research Institute for Obstetrics, Gynecology and Reproduction, 199034 St. Petersburg, Russia.; Antenatal Clinic No.26 Maternity Hospital No.10, 198259 St. Petersburg, Russia., Ivanova LA; Antenatal Clinic No.26 Maternity Hospital No.10, 198259 St. Petersburg, Russia., Eremeeva DR; II Obstetric Department Pathology of Pregnancy, V. F. Snegirev Maternity Hospital No.6, 192014 St. Petersburg, Russia., Zainulina MS; II Obstetric Department Pathology of Pregnancy, V. F. Snegirev Maternity Hospital No.6, 192014 St. Petersburg, Russia., Bespalova ON; Department of Genomic Medicine, D.O. Ott Research Institute for Obstetrics, Gynecology and Reproduction, 199034 St. Petersburg, Russia., Glotov AS; Department of Genomic Medicine, D.O. Ott Research Institute for Obstetrics, Gynecology and Reproduction, 199034 St. Petersburg, Russia.; Institute of Translational Biomedicine, St. Petersburg State University, 199034 St. Petersburg, Russia. |
| Abstrakt: |
Although circulating microRNAs (miRNAs) in maternal blood may play an important role in regulation of pregnancy progression and serve as non-invasive biomarkers for different gestation complications, little is known about their profile in blood during normally developing pregnancy. In this study we evaluated the miRNA profiles in paired plasma and serum samples from pregnant women without health or gestational abnormalities at three time points using high-throughput sequencing technology. Sequencing revealed that the percentage of miRNA reads in plasma and serum decreased by a third compared to first and second trimesters. We found two miRNAs in plasma (hsa-miR-7853-5p and hsa-miR-200c-3p) and 10 miRNAs in serum (hsa-miR-203a-5p, hsa-miR-495-3p, hsa-miR-4435, hsa-miR-340-5p, hsa-miR-4417, hsa-miR-1266-5p, hsa-miR-4494, hsa-miR-134-3p, hsa-miR-5008-5p, and hsa-miR-6756-5p), that exhibit level changes during pregnancy ( p -value adjusted < 0.05). In addition, we observed differences for 36 miRNAs between plasma and serum ( p -value adjusted < 0.05), which should be taken into consideration when comparing the results between studies performed using different biosample types. The results were verified by analysis of three miRNAs using qRT-PCR ( p < 0.05). The present study confirms that the circulating miRNA profile in blood changes during gestation. Our results set the basis for further investigation of molecular mechanisms, involved in regulation of pregnancy, and the search for biomarkers of gestation abnormalities. |
