Live Cell Poration by Au Nanostars to Probe Intracellular Molecular Composition with SERS.

Autor: Nikelshparg EI; Department of Biophysics, Biological Faculty, Moscow State University, 1-12 Leninskie Gory, 119991 Moscow, Russia.; Science Medical Center, Saratov State University, 83 Astrakhanskaya, 410012 Saratov, Russia., Prikhozhdenko ES; Science Medical Center, Saratov State University, 83 Astrakhanskaya, 410012 Saratov, Russia., Verkhovskii RA; Science Medical Center, Saratov State University, 83 Astrakhanskaya, 410012 Saratov, Russia., Atkin VS; Science Medical Center, Saratov State University, 83 Astrakhanskaya, 410012 Saratov, Russia., Khanadeev VA; Institute of Biochemistry and Physiology of Plants and Microorganisms, Russian Academy of Sciences, 13 Prospekt Entuziastov, 410049 Saratov, Russia.; Veterinary Medicine and Biotechnology Faculty, Saratov State Agrarian University, 1 Teatralnaya Square, 410012 Saratov, Russia., Khlebtsov BN; Science Medical Center, Saratov State University, 83 Astrakhanskaya, 410012 Saratov, Russia.; Institute of Biochemistry and Physiology of Plants and Microorganisms, Russian Academy of Sciences, 13 Prospekt Entuziastov, 410049 Saratov, Russia., Bratashov DN; Science Medical Center, Saratov State University, 83 Astrakhanskaya, 410012 Saratov, Russia.; Moscow Institute of Physics and Technology, 9 Institutskiy per., 141701 Dolgoprudny, Russia.
Jazyk: angličtina
Zdroj: Nanomaterials (Basel, Switzerland) [Nanomaterials (Basel)] 2021 Sep 30; Vol. 11 (10). Date of Electronic Publication: 2021 Sep 30.
DOI: 10.3390/nano11102588
Abstrakt: A new type of flat substrate has been used to visualize structures inside living cells by surface-enhanced Raman scattering (SERS) and to study biochemical processes within cells. The SERS substrate is formed by stabilized aggregates of gold nanostars on a glass microscope slide coated with a layer of poly (4-vinyl pyridine) polymer. This type of SERS substrate provides good cell adhesion and viability. Au nanostars' long tips can penetrate the cell membrane, allowing it to receive the SERS signal from biomolecules inside a living cell. The proposed nanostructured surfaces were tested to study, label-free, the distribution of various biomolecules in cell compartments.
Databáze: MEDLINE