| Autor: |
Beyi AF; Department of Veterinary Microbiology and Preventative Medicine, College of Veterinary Medicine, Iowa State University, Ames, IA 50011, USA., Brito-Goulart D; Department of Veterinary Microbiology and Preventative Medicine, College of Veterinary Medicine, Iowa State University, Ames, IA 50011, USA., Hawbecker T; College of Veterinary Medicine, Iowa State University, Ames, IA 50011, USA., Ruddell B; Department of Veterinary Microbiology and Preventative Medicine, College of Veterinary Medicine, Iowa State University, Ames, IA 50011, USA., Hassall A; Department of Veterinary Diagnostic and Production Animal Production, College of Veterinary Medicine, Iowa State University, Ames, IA 50011, USA., Dewell R; Center for Food Security/Public Health, College of Veterinary Medicine, Iowa State University, Ames, IA 50011, USA., Dewell G; Department of Veterinary Diagnostic and Production Animal Production, College of Veterinary Medicine, Iowa State University, Ames, IA 50011, USA., Sahin O; Department of Veterinary Diagnostic and Production Animal Production, College of Veterinary Medicine, Iowa State University, Ames, IA 50011, USA., Zhang Q; Department of Veterinary Microbiology and Preventative Medicine, College of Veterinary Medicine, Iowa State University, Ames, IA 50011, USA., Plummer PJ; Department of Veterinary Microbiology and Preventative Medicine, College of Veterinary Medicine, Iowa State University, Ames, IA 50011, USA.; Department of Veterinary Diagnostic and Production Animal Production, College of Veterinary Medicine, Iowa State University, Ames, IA 50011, USA.; National Institute of Antimicrobial Resistance Research and Education, Iowa State University, Ames, IA 50010, USA. |
| Abstrakt: |
Enrofloxacin is a fluoroquinolone drug used to prevent and control bovine respiratory disease (BRD) complex in multiple or single doses, ranging from 7.5 to 12.5 mg/kg body weight. Here, we examined the effects of high and low doses of a single subcutaneously injected enrofloxacin on gut microbiota and resistome in calves. Thirty-five calves sourced for this study were divided into five groups: control ( n = 7), two low dose groups ( n = 14, 7.5 mg/kg), and two high dose groups ( n = 14, 12.5 mg/kg). One group in the low and high dose groups was challenged with Mannheimia haemolytica to induce BRD. Both alpha and beta diversities were significantly different between pre- and post-treatment microbial communities ( q < 0.05). The high dose caused a shift in a larger number of genera than the low dose. Using metagenomic ProxiMeta Hi-C, 32 unique antimicrobial resistance genes (ARGs) conferring resistance to six antibiotic classes were detected with their reservoirs, and the high dose favored clonal expansion of ARG-carrying bacterial hosts. In conclusion, enrofloxacin treatment can alter fecal microbiota and resistome irrespective of its dose. Hi-C sequencing provides significant benefits for unlocking new insights into the ARG ecology of complex samples; however, limitations in sample size and sequencing depth suggest that further work is required to validate the findings. |
