| Autor: |
Kolouchová I; Department of Biotechnology, Faculty of Food and Biochemical Technology, University of Chemistry and Technology Prague, Technická 5, 16 628 Prague, Czech Republic., Timkina E; Department of Biotechnology, Faculty of Food and Biochemical Technology, University of Chemistry and Technology Prague, Technická 5, 16 628 Prague, Czech Republic., Maťátková O; Department of Biotechnology, Faculty of Food and Biochemical Technology, University of Chemistry and Technology Prague, Technická 5, 16 628 Prague, Czech Republic., Kyselová L; Research Institute of Brewing and Malting, Lípová 511, 120 44 Prague, Czech Republic., Řezanka T; Institute of Microbiology, The Czech Academy of Sciences, Vídeňská 1083, 142 20 Prague, Czech Republic. |
| Abstrakt: |
Background : Hopanoids modify plasma membrane properties in bacteria and are often compared to sterols that modulate membrane fluidity in eukaryotes. In some microorganisms, they can also allow adaptations to extreme environments. Methods : Hopanoids were identified by liquid chromatography-mass spectrometry in fourteen strains of thermophilic bacteria belonging to five genera, i.e., Alicyclobacillus, Brevibacillus, Geobacillus, Meiothermus , and Thermus . The bacteria were cultivated at temperatures from 42 to 70 °C. Results : Regardless of the source of origin, the strains have the same tendency to adapt the hopanoid content depending on the cultivation temperature. In the case of aminopentol, its content increases; aminotetrol does not show a significant change; and in the case of aminotriol the content decreases by almost a third. The content of bacteriohopanetetrol and bacteriohopanetetrol glycoside decreases with increasing temperature, while in the case of adenosylhopane the opposite trend was found. Conclusions : Changes in hopanoid content can be explained by increased biosynthesis, where adenosylhopane is the first intermediate in the biosynthesis of the hopanoid side chain. |
