| Autor: |
Mariani J; EPIGET LAB, Department of Clinical Sciences and Community Health, University of Milan, 20122 Milan, Italy., Iodice S; EPIGET LAB, Department of Clinical Sciences and Community Health, University of Milan, 20122 Milan, Italy., Cantone L; EPIGET LAB, Department of Clinical Sciences and Community Health, University of Milan, 20122 Milan, Italy., Solazzo G; EPIGET LAB, Department of Clinical Sciences and Community Health, University of Milan, 20122 Milan, Italy., Marraccini P; Department of Preventive Medicine, Fondazione IRCCS Ca' Granda Ospedale Maggiore Policlinico, 20122 Milan, Italy., Conforti E; Department of Preventive Medicine, Fondazione IRCCS Ca' Granda Ospedale Maggiore Policlinico, 20122 Milan, Italy., Bulsara PA; GlaxoSmithKline Consumer Healthcare, Warren, NJ 07059, USA., Lombardi MS; GlaxoSmithKline Consumer Healthcare, CH-1260 Nyon, Switzerland., Howlin RP; GlaxoSmithKline Consumer Healthcare, Weybridge KT13 0DE, UK., Bollati V; EPIGET LAB, Department of Clinical Sciences and Community Health, University of Milan, 20122 Milan, Italy., Ferrari L; EPIGET LAB, Department of Clinical Sciences and Community Health, University of Milan, 20122 Milan, Italy. |
| Abstrakt: |
Particulate matter (PM) exposure is linked to the worsening of respiratory conditions, including allergic rhinitis (AR), as it can trigger nasal and systemic inflammation. To unveil the underlying molecular mechanisms, we investigated the effects of PM exposure on the release of plasmatic extracellular vesicles (EV) and on the complex cross-talk between the host and the nasal microbiome. To this aim, we evaluated the effects of PM 10 and PM 2.5 exposures on both the bacteria-derived-EV portion (bEV) and the host-derived EVs (hEV), as well as on bacterial nasal microbiome (bNM) features in 26 AR patients and 24 matched healthy subjects (HS). In addition, we assessed the role exerted by the bNM as a modifier of PM effects on the complex EV signaling network in the paradigmatic context of AR. We observed that PM exposure differently affected EV release and bNM composition in HS compared to AR, thus potentially contributing to the molecular mechanisms underlying AR. The obtained results represent the first step towards the understanding of the complex signaling network linking external stimuli, bNM composition, and the immune risponse. |
