| Autor: |
Soljic V; Laboratory of Morphology, Department of Histology and Embryology, School of Medicine, University of Mostar, 88 000 Mostar, Bosnia and Herzegovina.; Faculty of Health Studies, University of Mostar, 88 000 Mostar, Bosnia and Herzegovina., Barbaric M; Laboratory of Morphology, Department of Histology and Embryology, School of Medicine, University of Mostar, 88 000 Mostar, Bosnia and Herzegovina., Vukoja M; Laboratory of Morphology, Department of Histology and Embryology, School of Medicine, University of Mostar, 88 000 Mostar, Bosnia and Herzegovina., Curlin M; Faculty of Health Studies, University of Mostar, 88 000 Mostar, Bosnia and Herzegovina., Orlovic Vlaho M; Faculty of Health Studies, University of Mostar, 88 000 Mostar, Bosnia and Herzegovina.; Department of Obstetrics, Gynecology University Clinical Hospital Mostar, 88 000 Mostar, Bosnia and Herzegovina., Cerni Obrdalj E; Department of Family Medicine, School of Medicine, University of Mostar, 88 000 Mostar, Bosnia and Herzegovina., Lasic Arapovic L; Laboratory of Morphology, Department of Histology and Embryology, School of Medicine, University of Mostar, 88 000 Mostar, Bosnia and Herzegovina.; Study Program of Dental Medicine, School of Medicine, University of Mostar, 88 000 Mostar, Bosnia and Herzegovina., Bevanda Glibo D; Department of Gastroenterology, University Hospital Mostar, 88000 Mostar, Bosnia and Herzegovina., Vukojevic K; Laboratory of Morphology, Department of Histology and Embryology, School of Medicine, University of Mostar, 88 000 Mostar, Bosnia and Herzegovina.; Department of Anatomy, Histology and Embryology, School of Medicine, University of Split, Šoltanska 2, 21 000 Split, Croatia. |
| Abstrakt: |
In our study, we aimed to establish expression of cytotoxic CD8 + T cells in the decidua basalis and the maternal peripheral blood (mPBL) of severe and mild preeclampsia (PE) and compare to healthy pregnancies. Decidual tissue and mPBL of 10 women with mild PE, 10 women with severe PE, and 20 age-matched healthy pregnancy controls were analyzed by double immunofluorescence and qPCR, respectively. By double immunofluorescence staining, we found a decreased total number of cells/mm 2 in decidua basalis of granulysin (GNLY) + ( p ˂ 0.0001), granzyme B (GzB) + ( p ˂ 0.0001), GzB + CD8 + ( p ˂ 0.0001), perforin (PRF1) + ( p ˂ 0.0001), and PRF1 + CD8 + ( p ˂ 0.01) in the severe PE compared to control group. Additionally, we noticed the trend of lower mRNA expression for GNLY, granzyme A (GZMA), GzB, and PRF1 in CD8 + T cells of mPBL in mild and severe PE, with the latter marker statistically decreased in severe PE ( p ˂ 0.001). Forkhead box P3 (FOXP3) mRNA in CD8 + T cells mPBL was increased in mild PE ( p ˂ 0.001) compared to controls. In conclusion, severe PE is characterized by altered expression of cytotoxic CD8 + T cells in decidua and mPBL, suggesting their role in pathophysiology of PE and fetal-maternal immune tolerance. |
