| Autor: |
Truong DTQ; National Institute of Veterinary Research, Hanoi 10000, Vietnam., Hounmanou YMG; Department of Veterinary and Animal Sciences, University of Copenhagen, 1870 Frederiksberg C, Denmark., Dang STT; National Institute of Veterinary Research, Hanoi 10000, Vietnam., Olsen JE; Department of Veterinary and Animal Sciences, University of Copenhagen, 1870 Frederiksberg C, Denmark., Truong GTH; National Institute of Veterinary Research, Hanoi 10000, Vietnam., Tran NT; National Institute of Veterinary Research, Hanoi 10000, Vietnam., Scheutz F; Department of Bacteria, Parasites and Fungi, Statens Serum Institute, 2300 Copenhagen S, Denmark., Dalsgaard A; Department of Veterinary and Animal Sciences, University of Copenhagen, 1870 Frederiksberg C, Denmark. |
| Jazyk: |
angličtina |
| Zdroj: |
Antibiotics (Basel, Switzerland) [Antibiotics (Basel)] 2021 Sep 25; Vol. 10 (10). Date of Electronic Publication: 2021 Sep 25. |
| DOI: |
10.3390/antibiotics10101165 |
| Abstrakt: |
We analyzed and compared genomes of Extended Spectrum Beta-Lactamase (ESBL)-producing Escherichia coli from pigs and pig farm workers at 116 farms in Vietnam. Analyses revealed the presence of bla CTX-M-55 , bla CTX-M-27 , bla CTX-M-15 , bla CTX-M-14 , bla CTX-M-3 , bla CTX-M-65 , bla CTX-M-24 , bla DHA-1 , and bla CMY2 in both hosts. Most strains from pigs contained quinolones ( qnr ) and colistin resistance genes ( mcr -1 and mcr -3). Isolates predominantly harbored more than one plasmid replicon and some harbored plasmid replicons on the same contigs as the ESBL genes. Five strains from farm workers of ST38 (2), ST69 (1), and ST1722 (2) were classified as either uropathogenic E. coli (UPEC HM )/extraintestinal pathogenic E. coli (ExPEC JJ ) or UPEC HM , and the remaining were genetically distinct commensals. A high heterogeneity was found among the ESBL-producing E. coli from pigs and workers, with most isolates belonging to unrelated phylogroups, serogroups, and sequence types with >4046 Single-Nucleotide Polymorphisms-(SNPs). In comparing the genomes of pig isolates to those from humans, it appeared that ESBL-producing E. coli in workers did not predominantly originate from pigs but were rather host-specific. Nevertheless, the occurrence of ESBL-producing E. coli carrying plasmid-mediated colistin and quinolone resistance genes in pigs could represent a potential source for horizontal transmission to humans through food rather than direct contact. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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