Piglet Gut and in-Barn Manure from Farms on a Raised without Antibiotics Program Display Reduced Antimicrobial Resistance but an Increased Prevalence of Pathogens.

Autor:	Chekabab SM; Food and Bioproduct Sciences, University of Saskatchewan, 51 Campus Drive, Saskatoon, SK S7N 5A8, Canada.; Prairie Swine Centre Inc., Box 21057, 2105-8th Street East, Saskatoon, SK S7H 5N9, Canada., Lawrence JR; Environment and Climate Change Canada, 11 Innovation Blvd., Saskatoon, SK S7N 3H5, Canada., Alvarado AC; Prairie Swine Centre Inc., Box 21057, 2105-8th Street East, Saskatoon, SK S7H 5N9, Canada.; Chemical and Biological Engineering, University of Saskatchewan, 57 Campus Drive, Saskatoon, SK S7N 5A9, Canada., Predicala BZ; Prairie Swine Centre Inc., Box 21057, 2105-8th Street East, Saskatoon, SK S7H 5N9, Canada.; Chemical and Biological Engineering, University of Saskatchewan, 57 Campus Drive, Saskatoon, SK S7N 5A9, Canada., Korber DR; Food and Bioproduct Sciences, University of Saskatchewan, 51 Campus Drive, Saskatoon, SK S7N 5A8, Canada.
Jazyk:	angličtina
Zdroj:	Antibiotics (Basel, Switzerland) [Antibiotics (Basel)] 2021 Sep 24; Vol. 10 (10). Date of Electronic Publication: 2021 Sep 24.
DOI:	10.3390/antibiotics10101152
Abstrakt:	In response to new stringent regulations in Canada regarding the use of antibiotics in animal production, many farms have implemented practices to produce animals that are raised without antibiotics (RWA) from birth to slaughter. This study aims to assess the impact of RWA production practices on reducing the actual total on-farm use of antibiotics, the occurrence of pathogens, and the prevalence of antimicrobial resistance (AMR). A 28-month longitudinal surveillance of farms that adopted the RWA program and conventional farms using antibiotics in accordance with the new regulations (non-RWA) was conducted by collecting fecal samples from 6-week-old pigs and composite manure from the barn over six time points and applying whole-genome sequencing (WGS) to assess the prevalence of AMR genes as well as the abundance of pathogens. Analysis of in-barn drug use records confirmed the decreased consumption of antibiotics in RWA barns compared to non-RWA barns. WGS analyses revealed that RWA barns had reduced the frequency of AMR genes in piglet feces and in-barn manure. However, metagenomic analyses showed that RWA barns had a significant increase in the frequency of pathogenic Firmicutes in fecal samples and pathogenic Proteobacteria in barn manure samples.
Databáze:	MEDLINE
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