Efficacy and Cost-Effectiveness of Phenotyping for Rice Resistance and Tolerance to Planthoppers.

Autor: Horgan FG; EcoLaVerna Integral Restoration Ecology, Bridestown, Kildinan, T56 P499 County Cork, Ireland.; Escuela de Agronomía, Facultad de Ciencias Agrarias y Forestales, Universidad Católica del Maule, Casilla 7-D, Curicó, Chile., Mundaca EA; Escuela de Agronomía, Facultad de Ciencias Agrarias y Forestales, Universidad Católica del Maule, Casilla 7-D, Curicó, Chile., Quintana R; International Rice Research Institute, DAPO Box 7777, Metro Manila 1301, Philippines., Naredo AI; International Rice Research Institute, DAPO Box 7777, Metro Manila 1301, Philippines., Almazan MLP; International Rice Research Institute, DAPO Box 7777, Metro Manila 1301, Philippines., Bernal CC; International Rice Research Institute, DAPO Box 7777, Metro Manila 1301, Philippines.
Jazyk: angličtina
Zdroj: Insects [Insects] 2021 Sep 22; Vol. 12 (10). Date of Electronic Publication: 2021 Sep 22.
DOI: 10.3390/insects12100847
Abstrakt: The standard seedling seedbox test (SSST) is the most prevalent phenotyping test in research on the genetics and breeding of planthopper-resistant rice. Using 16 rice lines that included plants susceptible, resistant and tolerant to the brown planthopper ( Nilaparvata lugens ), we compared the SSST to modified seedling seedbox tests (MSSTs) and the days-to-wilt (DTW) test. We also conducted a series of performance tests to assess nymph survival and development; adult longevity and egg-laying; egg survival; honeydew production; and plant weight loss. We also assessed the relative costs of the different phenotyping tests to better recommend test protocols that are suitable for high-throughput phenotyping. The SSST was found to be highly robust but fails to identify late-stage resistance; tolerance; or ovicidal responses. MSSTs improved phenotyping by identifying plants with low damage from planthoppers at later growth stages. Herbivore performance tests such as population or biomass build-up tests reduce space requirements and reduce setup and evaluation costs compared with bulk tests. They can also facilitate the assessment of plant tolerance; albeit with added costs. The DTW test most clearly segregates resistant and susceptible plants, thereby facilitating gene discovery and marker-assisted selection. We recommend that bulk testing be improved by switching from the SSST to a suitable MSST and that donor variety and pre-release lines be assessed for the nature of rice-planthopper interactions using biomass build-up tests-including the DTW test.
Databáze: MEDLINE
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