| Autor: |
Peng YC; Biotechnology Industry Master and PhD Program, Chang Gung University, Taoyuan 33302, Taiwan., Cheng CH; Tst Biomedical Electronics Co., Ltd., Taoyuan 324, Taiwan., Yatsuda H; Tst Biomedical Electronics Co., Ltd., Taoyuan 324, Taiwan., Liu SH; Tst Biomedical Electronics Co., Ltd., Taoyuan 324, Taiwan., Liu SJ; National Institute of Infectious Diseases and Vaccinology, National Health Research Institutes, Miaoli 35053, Taiwan., Kogai T; Tst Biomedical Electronics Co., Ltd., Taoyuan 324, Taiwan.; Japan Radio Co., Ltd., Saitama 356-8510, Japan., Kuo CY; Division of Pediatric Infectious Diseases, Department of Pediatrics, Chang Gung Memorial and Children's Hospital, Linkou 33305, Taiwan., Wang RYL; Biotechnology Industry Master and PhD Program, Chang Gung University, Taoyuan 33302, Taiwan.; Division of Pediatric Infectious Diseases, Department of Pediatrics, Chang Gung Memorial and Children's Hospital, Linkou 33305, Taiwan.; Department of Biomedical Sciences, College of Medicine, Chang Gung University, Taoyuan 33302, Taiwan. |
| Abstrakt: |
Since the Coronavirus disease 2019 (COVID-19) pandemic outbreak, many methods have been used to detect antigens or antibodies to severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), including viral culture, nucleic acid test, and immunoassay. The shear-horizontal surface acoustic wave (SH-SAW) biosensor is a novel pathogen detection platform with the advantages of high sensitivity and short detection time. The objective of this study is to develop a SH-SAW biosensor to detect the anti-SARS-CoV-2 nucleocapsid antibody. The rabbit sera collected from rabbits on different days after SARS-CoV-2 N protein injection were evaluated by SH-SAW biosensor and enzyme-linked immunosorbent assay (ELISA). The results showed that the SH-SAW biosensor achieved a high correlation coefficient (R = 0.9997) with different concentrations (34.375-1100 ng/mL) of the "spike-in" anti-N protein antibodies. Compared to ELISA, the SH-SAW biosensor has better sensitivity and can detect anti-N protein IgG signals earlier than ELISA on day 6 ( p < 0.05). Overall, in this study, we demonstrated that the SH-SAW biosensor is a promising platform for rapid in vitro diagnostic (IVD) testing, especially for antigen or antibody testing. |
