Effects of albumin-bound nitrosyl iron complex with thiosulfate ligands on lipid peroxidation and activities of mitochondrial enzymes in vitro.

Autor: Faingold II; Institute of Problems of Chemical Physics of the RAS, Chernogolovka, Moscow Region, Russian Federation., Poletaeva DA; Institute of Problems of Chemical Physics of the RAS, Chernogolovka, Moscow Region, Russian Federation. Electronic address: dapol@icp.ac.ru., Soldatova YV; Institute of Problems of Chemical Physics of the RAS, Chernogolovka, Moscow Region, Russian Federation., Smolina AV; Institute of Problems of Chemical Physics of the RAS, Chernogolovka, Moscow Region, Russian Federation., Pokidova OV; Institute of Problems of Chemical Physics of the RAS, Chernogolovka, Moscow Region, Russian Federation., Kulikov AV; Institute of Problems of Chemical Physics of the RAS, Chernogolovka, Moscow Region, Russian Federation., Sanina NA; Institute of Problems of Chemical Physics of the RAS, Chernogolovka, Moscow Region, Russian Federation; Medicinal Chemistry Research and Education Center, Moscow Region State University, Mytishchy, Moscow region, Russian Federation., Kotelnikova RA; Institute of Problems of Chemical Physics of the RAS, Chernogolovka, Moscow Region, Russian Federation.
Jazyk: angličtina
Zdroj: Nitric oxide : biology and chemistry [Nitric Oxide] 2021 Dec 01; Vol. 117, pp. 46-52. Date of Electronic Publication: 2021 Oct 20.
DOI: 10.1016/j.niox.2021.10.002
Abstrakt: Nitric oxide (NO) mediates diverse physiological processes in living organisms. Small molecular NO donors usually lack stability and have a short half-life in human tissues, limiting the therapeutic application. The anionic tetranitrosyl iron complex with thiosulfate ligands (TNIC) is one of the most promising NO donors. This study shows that bovine serum albumin (BSA) can effectively stabilize the TNIC complex under aerobic (physiological) conditions, which contributes to its prolonged action as NO donor. Our results demonstrated that TNIC-BSA inhibits formation of TBARS - standard biomarker for the lipid peroxidation induced oxidative stress. Also, it was found that TNIC-BSA inhibits the catalytic activity of mitochondrial membrane-bound enzymes: cytochrome c oxidase and monoamine oxidase A. Together, these results demonstrate that, stabilization of TNIC with BSA opens up the possibility of its practical application in chemotherapy of socially significant diseases.
(Copyright © 2021 Elsevier Inc. All rights reserved.)
Databáze: MEDLINE
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