Chronic bladder catheterization for precise urine collection in awake mice.

Autor: Andreev-Andrievskiy AA; M.V. Lomonosov Moscow State University, Biology Faculty, 119991 Moscow, 1-12 Leninskie Gory, Russia; Institute of biomedical problems, Russian Academy of Science, 123007 Moscow, 76A Khoroshevskoe Shosse, Russia; MSU Institute for Mitoengineering, LLC, 119991 Moscow, 1-73 Leninskie Gory, Russia. Electronic address: aandrievsky@gmail.com., Lagereva EA; Institute of biomedical problems, Russian Academy of Science, 123007 Moscow, 76A Khoroshevskoe Shosse, Russia; MSU Institute for Mitoengineering, LLC, 119991 Moscow, 1-73 Leninskie Gory, Russia., Pankova NV; Institute of biomedical problems, Russian Academy of Science, 123007 Moscow, 76A Khoroshevskoe Shosse, Russia; MSU Institute for Mitoengineering, LLC, 119991 Moscow, 1-73 Leninskie Gory, Russia., Mashkin MA; Institute of biomedical problems, Russian Academy of Science, 123007 Moscow, 76A Khoroshevskoe Shosse, Russia; MSU Institute for Mitoengineering, LLC, 119991 Moscow, 1-73 Leninskie Gory, Russia., Manskikh VN; MSU Institute for Mitoengineering, LLC, 119991 Moscow, 1-73 Leninskie Gory, Russia; A.N. Belozerskiy Institute of Physico-Chemical Biology, 119991 Moscow, 1-40 Leninskie Gory, Russia., Frolova OY; MSU Institute for Mitoengineering, LLC, 119991 Moscow, 1-73 Leninskie Gory, Russia., Fadeeva OV; MSU Institute for Mitoengineering, LLC, 119991 Moscow, 1-73 Leninskie Gory, Russia., Telyatnikova EV; MSU Institute for Mitoengineering, LLC, 119991 Moscow, 1-73 Leninskie Gory, Russia.
Jazyk: angličtina
Zdroj: Journal of pharmacological and toxicological methods [J Pharmacol Toxicol Methods] 2022 Jan-Feb; Vol. 113, pp. 107128. Date of Electronic Publication: 2021 Oct 19.
DOI: 10.1016/j.vascn.2021.107128
Abstrakt: Metabolic chambers are routinely used for urine collection in rodents. In mice, due to small urination volume, evaporation in the metabolic chambers (≈50%) distorts diuresis and urinalysis parameters. We have developed a new technique of bladder catheterization enabling long-term accurate and contamination-free urine collection in awake male and female mice for 30 days or longer. Daily diuresis in catheterized mice was twice higher as compared to metabolic cages. The twofold difference in urine recovery was preserved when the circadian variation of diuresis, the effects of furosemide, desmopressin and water load were estimated using the two techniques. Urine osmolarity, urinalysis, and microbiological parameters evidence higher quality of the catheter-collected urine. Using phenol red, we demonstrate utility of our technique for pharmacokinetic studies. 30 days after the surgery the catheters were patent and had minimal impact on the animals' heath. Bladder catheterization is a useful tool for physiological, pharmacological, and toxicological studies.
(Copyright © 2021 Elsevier Inc. All rights reserved.)
Databáze: MEDLINE
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