Insulin stimulates β-alanine uptake in skeletal muscle cells in vitro.

Autor: Santos L; Musculoskeletal Physiology Research Group, Sport, Health and Performance Enhancement Research Centre, Nottingham Trent University, Nottingham, UK., Gonçalves LS; Applied Physiology and Nutrition Research GroupRheumatology DivisionFaculdade de Medicina FMUSPEscola de Educação Física E Esporte, Universidade de São Paulo, São Paulo, SP, Brazil., Bagheri-Hanei S; College of Engineering and Physical Science, Aston University, Birmingham, B4 7ET, UK., Möller GB; Applied Physiology and Nutrition Research GroupRheumatology DivisionFaculdade de Medicina FMUSPEscola de Educação Física E Esporte, Universidade de São Paulo, São Paulo, SP, Brazil., Sale C; Musculoskeletal Physiology Research Group, Sport, Health and Performance Enhancement Research Centre, Nottingham Trent University, Nottingham, UK., James RM; Musculoskeletal Physiology Research Group, Sport, Health and Performance Enhancement Research Centre, Nottingham Trent University, Nottingham, UK., Artioli GG; Department of Life Sciences, Manchester Metropolitan University, John Dalton Building, Manchester, M1 5GD, UK. G.Giannini.Artioli@mmu.ac.uk.
Jazyk: angličtina
Zdroj: Amino acids [Amino Acids] 2021 Nov; Vol. 53 (11), pp. 1763-1766. Date of Electronic Publication: 2021 Oct 21.
DOI: 10.1007/s00726-021-03090-9
Abstrakt: We evaluated whether insulin could stimulate β-alanine uptake by skeletal muscle cells in vitro. Mouse myoblasts (C2C12) (n = 3 wells per condition) were cultured with β-alanine (350 or 700 µmol·L -1 ), with insulin (100 µU·mL -1 ) either added to the media or not. Insulin stimulated the β-alanine uptake at the lower (350 µmol·L -1 ) but not higher (700 µmol·L -1 ) β-alanine concentration in culture medium, indicating that transporter saturation might blunt the stimulatory effects of insulin.
(© 2021. The Author(s).)
Databáze: MEDLINE
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