Development and characterization of a new swine model of invasive pneumococcal pneumonia.

Autor:	Amaro R; Division of Animal Experimentation, Pneumology Department, Hospital Clinic, Barcelona, Spain.; Institut d'Investigacions Biomèdiques August Pi i Sunyer (IDIBAPS), Barcelona, Spain.; Centro de Investigación Biomedica En Red-Enfermedades Respiratorias (CIBERES), Barcelona, Spain., Li Bassi G; Division of Animal Experimentation, Pneumology Department, Hospital Clinic, Barcelona, Spain.; Institut d'Investigacions Biomèdiques August Pi i Sunyer (IDIBAPS), Barcelona, Spain.; Critical Care Research Group, Prince Charles Hospital, Chermside, Australia.; University of Queensland, Brisbane, Australia., Motos A; Division of Animal Experimentation, Pneumology Department, Hospital Clinic, Barcelona, Spain.; Institut d'Investigacions Biomèdiques August Pi i Sunyer (IDIBAPS), Barcelona, Spain.; Centro de Investigación Biomedica En Red-Enfermedades Respiratorias (CIBERES), Barcelona, Spain.; University of Barcelona, Barcelona, Spain., Fernandez-Barat L; Division of Animal Experimentation, Pneumology Department, Hospital Clinic, Barcelona, Spain.; Institut d'Investigacions Biomèdiques August Pi i Sunyer (IDIBAPS), Barcelona, Spain.; Centro de Investigación Biomedica En Red-Enfermedades Respiratorias (CIBERES), Barcelona, Spain.; University of Barcelona, Barcelona, Spain., Aguilera Xiol E; Division of Animal Experimentation, Pneumology Department, Hospital Clinic, Barcelona, Spain.; Institut d'Investigacions Biomèdiques August Pi i Sunyer (IDIBAPS), Barcelona, Spain., Rigol M; Division of Animal Experimentation, Pneumology Department, Hospital Clinic, Barcelona, Spain.; Institut d'Investigacions Biomèdiques August Pi i Sunyer (IDIBAPS), Barcelona, Spain., Frigola G; Department of Pathology, Hospital Clinic, Barcelona, Spain., Travierso C; Division of Animal Experimentation, Pneumology Department, Hospital Clinic, Barcelona, Spain.; Division of Pneumology, ASST Rhodense 'Guido Salvini' Hospital, Garbagnate Milanese, Italy., Bobi J; Division of Animal Experimentation, Pneumology Department, Hospital Clinic, Barcelona, Spain.; Institut d'Investigacions Biomèdiques August Pi i Sunyer (IDIBAPS), Barcelona, Spain., Pagliara F; Department of Surgical Sciences and Integrated Diagnostics (DISC), IRCCS AOU San Martino IST, Genova, Italy.; San Martino Policlinico Hospital, IRCCS for Oncology and Neurosciences, Genoa, Italy., Carbonara M; Division of Animal Experimentation, Pneumology Department, Hospital Clinic, Barcelona, Spain.; University of Milan, Milan, Italy., Comaru T; Division of Animal Experimentation, Pneumology Department, Hospital Clinic, Barcelona, Spain.; Instituto Federal Farroupilha, Santo Ângelo, Brasil (sponsored by CNPq), Santo Ângelo, Brazil., Chiurazzi C; Division of Animal Experimentation, Pneumology Department, Hospital Clinic, Barcelona, Spain.; Humanitas Clinical and Research Center, Rozzano, Milan, Italy., Yang M; Division of Animal Experimentation, Pneumology Department, Hospital Clinic, Barcelona, Spain.; Institut d'Investigacions Biomèdiques August Pi i Sunyer (IDIBAPS), Barcelona, Spain., Yang H; Division of Animal Experimentation, Pneumology Department, Hospital Clinic, Barcelona, Spain.; Institut d'Investigacions Biomèdiques August Pi i Sunyer (IDIBAPS), Barcelona, Spain., Arrieta M; Division of Animal Experimentation, Pneumology Department, Hospital Clinic, Barcelona, Spain., Marti JD; Division of Animal Experimentation, Pneumology Department, Hospital Clinic, Barcelona, Spain., De Rosa F; Division of Animal Experimentation, Pneumology Department, Hospital Clinic, Barcelona, Spain., Saco MA; Department of Pathology, Hospital Clinic, Barcelona, Spain., Rinaudo M; Division of Animal Experimentation, Pneumology Department, Hospital Clinic, Barcelona, Spain., Terraneo S; Division of Animal Experimentation, Pneumology Department, Hospital Clinic, Barcelona, Spain., Schultz MJ; Department of Intensive Care, Academic Medical Center, University of Amsterdam, Amsterdam, Netherlands., Nicolau DP; Center for Anti-Infective Research & Development, Hartford Hospital, Hartford, CT, USA., Artigas A; Centro de Investigación Biomedica En Red-Enfermedades Respiratorias (CIBERES), Barcelona, Spain.; Pathophysiological Laboratory, Institut de Investigacion Parc Tauli, Corporació Sanitaria Universitaria Parc Tauli, Autonomous University of Barcelona, Sabadell, Barcelona, Spain., Ramirez J; Department of Pathology, Hospital Clinic, Barcelona, Spain., Torres A; Division of Animal Experimentation, Pneumology Department, Hospital Clinic, Barcelona, Spain. atorres@clinic.cat.; Institut d'Investigacions Biomèdiques August Pi i Sunyer (IDIBAPS), Barcelona, Spain. atorres@clinic.cat.; Centro de Investigación Biomedica En Red-Enfermedades Respiratorias (CIBERES), Barcelona, Spain. atorres@clinic.cat.; University of Barcelona, Barcelona, Spain. atorres@clinic.cat.
Jazyk:	angličtina
Zdroj:	Lab animal [Lab Anim (NY)] 2021 Nov; Vol. 50 (11), pp. 327-335. Date of Electronic Publication: 2021 Oct 21.
DOI:	10.1038/s41684-021-00876-y
Abstrakt:	Streptococcus pneumoniae is the most common microbial cause of community-acquired pneumonia. Currently, there are no available models of severe pneumococcal pneumonia in mechanically ventilated animals to mimic clinical conditions of critically ill patients. We studied endogenous pulmonary flora in 4 healthy pigs and in an additional 10 pigs in which we intra-bronchially instilled S. pneumoniae serotype 19 A, characterized by its resistance to penicillin, macrolides and tetracyclines. The pigs underwent ventilation for 72 h. All pigs that were not challenged with S. pneumoniae completed the 72-h study, whereas 30% of infected pigs did not. At 24 h, we clinically confirmed pneumonia in the infected pigs; upon necropsy, we sampled lung tissue for microbiological/histological confirmation of pneumococcal pneumonia. In control pigs, Streptococcus suis and Staphylococcus aureus were the most commonly encountered pathogens, and their lung tissue mean ± s.e.m. concentration was 7.94 ± 20 c.f.u./g. In infected pigs, S. pneumoniae was found in the lungs of all pigs (mean ± s.e.m. pulmonary concentration of 1.26 × 10 5 ± 2 × 10 2 c.f.u./g). Bacteremia was found in 50% of infected pigs. Pneumococcal pneumonia was confirmed in all infected pigs at 24 h. Pneumonia was associated with thrombocytopenia, an increase in prothrombin time, cardiac output and vasopressor dependency index and a decrease in systemic vascular resistance. Upon necropsy, microbiological/histological pneumococcal pneumonia was confirmed in 8 of 10 pigs. We have therefore developed a novel model of penicillin- and macrolide-resistant pneumococcal pneumonia in mechanically ventilated pigs with bacteremia and severe hemodynamic compromise. The model could prove valuable for appraising the pathogenesis of pneumococcal pneumonia, the effects associated with macrolide resistance and the outcomes related to the use of new diagnostic strategies and antibiotic or complementary therapies. (© 2021. The Author(s), under exclusive licence to Springer Nature America, Inc.)
Databáze:	MEDLINE
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