Alleviation of Cadmium Chloride-Induced Acute Genotoxicity, Mitochondrial DNA Disruption, and ROS Generation by Chocolate Coadministration in Mice Liver and Kidney Tissues.

Autor: Mohamed HRH; Zoology Department, Faculty of Science, Cairo University, Giza, Egypt. hananeeyra@cu.edu.eg.
Jazyk: angličtina
Zdroj: Biological trace element research [Biol Trace Elem Res] 2022 Aug; Vol. 200 (8), pp. 3750-3761. Date of Electronic Publication: 2021 Oct 21.
DOI: 10.1007/s12011-021-02981-y
Abstrakt: Increased human exposure to cadmium compounds through ingesting contaminated food, water, and medications causes negative long-term health effects, which has led to the focus of recent researches on finding natural antioxidants to mitigate cadmium-induced toxicity. Therefore, the current study was undertaken to estimate the possible ameliorative effect of chocolate coadministration on acute cadmium chloride (CdCl 2 )-induced genomic instability and mitochondrial DNA damage in mice liver and kidney tissues. Concurrent administration of chocolate with CdCl 2 dramatically decreased the DNA damage level and the number of apoptotic and necrotic cells compared to mice given CdCl 2 alone. Extra-production of reactive oxygen species and increased expression of inducible nitric oxide synthase and heat shock proteins genes caused by CdCl 2 administration were also highly decreased after chocolate coadministration. Conversely, chocolate coadministration restored the integrity of the mitochondrial membrane potential disrupted by CdCl 2 administration, as well as the mitochondrial DNA copy number and expression level of heme oxygenase-1 gene were significantly upregulated after chocolate coadministration with CdCl 2 . Thus, it was concluded that the coadministration of chocolate alleviated CdCl 2 -induced genomic instability and mitochondrial DNA damage through its antioxidative and free radical scavenging capabilities, making chocolate a promising ameliorative product and recommended for inclusion in the daily human diet.
(© 2021. The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature.)
Databáze: MEDLINE
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