Children Environmentally Exposed to Agrochemicals in Rural Areas Present Changes in Oxidative Status and DNA Damage.

Autor: Nascimento S; Laboratory of Toxicology (LATOX), Department of Analysis, Faculty of Pharmacy, Federal University of Rio Grande Do Sul, Rua São Luis 150-Anexo II, Santa Cecília, Porto Alegre, RS, CEP, 90610-000, Brazil.; Graduate Program in Pharmaceutical Sciences, Federal University of Rio Grande Do Sul, Porto Alegre, RS, Brazil., Brucker N; Graduate Program in Pharmacology, Federal University of Santa Maria, Santa Maria, RS, Brazil., Göethel G; Laboratory of Toxicology (LATOX), Department of Analysis, Faculty of Pharmacy, Federal University of Rio Grande Do Sul, Rua São Luis 150-Anexo II, Santa Cecília, Porto Alegre, RS, CEP, 90610-000, Brazil.; Graduate Program in Pharmaceutical Sciences, Federal University of Rio Grande Do Sul, Porto Alegre, RS, Brazil., Sauer E; Laboratory of Toxicology (LATOX), Department of Analysis, Faculty of Pharmacy, Federal University of Rio Grande Do Sul, Rua São Luis 150-Anexo II, Santa Cecília, Porto Alegre, RS, CEP, 90610-000, Brazil., Peruzzi C; Laboratory of Toxicology (LATOX), Department of Analysis, Faculty of Pharmacy, Federal University of Rio Grande Do Sul, Rua São Luis 150-Anexo II, Santa Cecília, Porto Alegre, RS, CEP, 90610-000, Brazil.; Graduate Program in Pharmaceutical Sciences, Federal University of Rio Grande Do Sul, Porto Alegre, RS, Brazil., Gauer B; Laboratory of Toxicology (LATOX), Department of Analysis, Faculty of Pharmacy, Federal University of Rio Grande Do Sul, Rua São Luis 150-Anexo II, Santa Cecília, Porto Alegre, RS, CEP, 90610-000, Brazil., Tureta E; Laboratory of Toxicology (LATOX), Department of Analysis, Faculty of Pharmacy, Federal University of Rio Grande Do Sul, Rua São Luis 150-Anexo II, Santa Cecília, Porto Alegre, RS, CEP, 90610-000, Brazil., Flesch I; Laboratory of Toxicology (LATOX), Department of Analysis, Faculty of Pharmacy, Federal University of Rio Grande Do Sul, Rua São Luis 150-Anexo II, Santa Cecília, Porto Alegre, RS, CEP, 90610-000, Brazil., Cestonaro L; Laboratory of Toxicology (LATOX), Department of Analysis, Faculty of Pharmacy, Federal University of Rio Grande Do Sul, Rua São Luis 150-Anexo II, Santa Cecília, Porto Alegre, RS, CEP, 90610-000, Brazil.; Graduate Program in Pharmaceutical Sciences, Federal University of Rio Grande Do Sul, Porto Alegre, RS, Brazil., Pierre TS; Department of Chemistry, Pontifícia Universidade Católica Do Rio de Janeiro, (PUC-Rio), Rio de Janeiro, RJ, Brazil., Gioda A; Department of Chemistry, Pontifícia Universidade Católica Do Rio de Janeiro, (PUC-Rio), Rio de Janeiro, RJ, Brazil., Garcia SC; Laboratory of Toxicology (LATOX), Department of Analysis, Faculty of Pharmacy, Federal University of Rio Grande Do Sul, Rua São Luis 150-Anexo II, Santa Cecília, Porto Alegre, RS, CEP, 90610-000, Brazil. solange.garcia@ufrgs.br.; Graduate Program in Pharmaceutical Sciences, Federal University of Rio Grande Do Sul, Porto Alegre, RS, Brazil. solange.garcia@ufrgs.br.
Jazyk: angličtina
Zdroj: Biological trace element research [Biol Trace Elem Res] 2022 Aug; Vol. 200 (8), pp. 3511-3518. Date of Electronic Publication: 2021 Oct 20.
DOI: 10.1007/s12011-021-02950-5
Abstrakt: Rural children are exposed to several chemicals. This study evaluated the environmental co-exposure of rural children to cholinesterase inhibitor insecticides and metals/metalloids, and the resulting oxidative stress and DNA damage. Seventy-two children (5 to 16 years old) were studied at two different moments: period 1, when agrochemicals were less used, and period 2, when agrochemicals were extensively used in agriculture. Biomonitoring was performed by evaluating butyrylcholinesterase (BuChE) activity in serum; arsenic (As), chromium (Cr), lead (Pb), and nickel (Ni) levels in blood; malondialdehyde (MDA) in plasma; glutathione peroxidase (GSH-Px) and glutathione S-transferase (GST) activities in whole blood; non-protein thiol levels in erythrocytes; and micronuclei (MN) assay in exfoliated buccal cells. Cr and As levels were higher than the reference values in both periods, and Ni levels were higher than the reference values in period 2 alone. BuChE activity was inhibited in period 2 compared with period 1. In period 2, there was an increase in endogenous antioxidants and a decrease in MDA, probably demonstrating a compensatory mechanism as a response to increasing xenobiotics. Also in period 2, the MN frequency increased and BuChE and As were positively associated, suggesting co-exposure. On the other hand, in period 1, it was observed that Cr, Ni, and Pb blood levels were negatively associated with GSH-Px and GST, while MDA was positively associated with As levels. Our findings demonstrated an imbalance in endogenous antioxidants, contributing to genotoxicity and lipoperoxidation, probably in response to exposure to xenobiotics, especially carcinogenic elements (Cr, As, and Ni).
(© 2021. The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature.)
Databáze: MEDLINE
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