Cell culture of the normal human mammary gland cultivated in monolayer - A mini systematic review.

Autor: de Almeida MHR; Medial Undergraduate Student, Department of Health Sciences, Federal University of Santa Catarina, Campus Araranguá, 88906-072 Araranguá, SC, Brazil. Electronic address: m.almeida@grad.ufsc.br., Bortolotto GDS; Laboratory Technician, Department of Health Sciences, Federal University of Santa Catarina, Campus Araranguá, 88906-072 Araranguá, SC, Brazil. Electronic address: geovanasavi@gmail.com., Dutra RC; Professor, Department of Health Sciences, Federal University of Santa Catarina, Campus Araranguá, 88906-072 Araranguá, SC, Brazil. Electronic address: rafaelcdutra@gmail.com., Guimarães GN; Laboratory Technician, Department of Biosciences, State University of Campinas, Campus Piracicaba, 13414-903 Piracicaba, SP, Brazil. Electronic address: gu_narvaes@yahoo.com.br., Felipetti FA; Professor, Department of Health Sciences, Federal University of Santa Catarina, Campus Araranguá, 88906-072 Araranguá, SC, Brazil. Electronic address: fafelipetti@gmail.com.
Jazyk: angličtina
Zdroj: Acta histochemica [Acta Histochem] 2021 Dec; Vol. 123 (8), pp. 151798. Date of Electronic Publication: 2021 Oct 16.
DOI: 10.1016/j.acthis.2021.151798
Abstrakt: The mammary glands are constituted of different cell types. For example, the epithelial cells appear as the target in many studies since they produce and secrete milk during lactation and are the origin of many human breast cancers. Mammary gland biology is characterized by dynamic tissue growth, function and regression phases, which are understood mainly due to tissue culture studies. Cell culture is probably one of the most used in vitro scientific models, and the most common research model is still the two-dimensional (2D) culture system. Different approaches and conditions have been tested and used to improve the isolation, growth, yield and maintenance of viability of mammary gland cells. Therefore, our study aimed to explore and summarize the cell culture techniques with normal human mammary gland cells cultured in a monolayer. A search strategy was conducted using the electronic databases 'PubMed', 'Scopus' and 'Virtual Health Library'. The search was carried out using the keywords 'cell culture' and 'mammary gland' and 'human'. The main search was carried out by two authors between July and August 2021. In addition, we performed a review matrix elaborated in a spreadsheet to organize and systematize information about each article for inclusion. A total of 11 studies were included in the review and have conducted qualitative analyses on them. Although studies of these cells have been reported since the 1970 s, most found are from the last decade and are largely carried out in the USA. In addition, it was possible to verify the Human Mammary Epithelial Cells (HMEC) primary culture obtained from breast surgery as the main cell type studied. These cells are cultivated in Dulbecco's Modified Eagle Medium (DMEM) and M87A medium with diverse supplements. Finally, there was a diversity in the use of dissociation reagents and a lack of information about cryopreservation. We have observed detailed methodological information about these study models, which would propose further investigations.
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Databáze: MEDLINE
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