Genome features and antibiotic resistance of Pseudomonas aeruginosa strains isolated in patients with cystic fibrosis in the Russian Federation.

Autor:	Bocharova YA; Pirogov Russian National Research Medical University., Savinova TA; Pirogov Russian National Research Medical University., Lyamin AV; Samara State Medical University., Kondratenko OV; Samara State Medical University., Polikarpova SV; Filatov Municipal Clinical Hospital., Zhilina SV; Morozov City Children's Clinical Hospital., Fedorova NI; Pirogov Russian National Research Medical University., Semykin SY; Pirogov Russian National Research Medical University., Chaplin AV; Pirogov Russian National Research Medical University., Korostin DO; Pirogov Russian National Research Medical University., Mayansky NA; Pirogov Russian National Research Medical University., Chebotar IV; Pirogov Russian National Research Medical University.
Jazyk:	angličtina
Zdroj:	Klinicheskaia laboratornaia diagnostika [Klin Lab Diagn] 2021 Oct 18; Vol. 66 (10), pp. 629-634.
DOI:	10.51620/0869-2084-2021-66-10-629-634
Abstrakt:	Cystic fibrosis (CF) is a common genetic disease, manifested by airway obstruction and chronic respiratory infection. The most prevalent infectious agent in airways of CF patients is Pseudomonas aeruginosa. This study aimed to determine sequence-types, antimicrobial resistance phenotypes and genes defining adaptive antibiotic resistance in P. aeruginosa isolates recovered from CF patients in Russia. In total, 84 P. aeruginosa strains from 64 CF patients were analyzed. Susceptibility to antibiotics was determined by disk diffusion test. Whole-genome sequencing (WGS) was performed on MGISEQ-2000 platform. SPAdes software, Galaxy, ResFinder, PubMLST were used for analysis of WGS data. Examined P. aeruginosa isolates belonged to 53 different sequence-types (STs), including 6 new STs. High-risk epidemic clone ST235 (10%) and clonal CF P. aeruginosa strains ST17, ST242, ST274 (7%) were detected. Non-susceptibility to ticarcillin-clavulanate, cefepime, imipenem was observed in 63%, 12% and 25% of isolates, respectively; to tobramycin - in 24%, to amikacin - in 35%; to ciprofloxacin, levofloxacin - in 35% and 57% of strains, respectively. Multidrug-resistant phenotype was detected in 18% of isolates. In examined strains, genes of beta-lactamases VIM-2 (5 ST235 strains), VEB-1 (two ST2592 strains), GES-1 (1 ST235 strain), PER-1 (1 ST235 strain) were found. Ciprofloxacin-modifying enzyme CrpP gene was detected in 67% of isolates, aminoglycoside-modifying enzymes AAD, ANT, AAC genes - in 7%, 4%, 12% of strains, respectively. P. aeruginosa isolates from CF patients in Russia demonstrate a high clonal diversity, which is similar to other P. aeruginosa infections. The isolates of high-risk clone and clonal CF P. aeruginosa strains are detected. Competing Interests: The authors declare no conflict of interest.
Databáze:	MEDLINE
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