No association between cataract surgery and mitochondrial DNA damage with age-related macular degeneration in human donor eyes.

Autor: Armbrust KR; Department of Ophthalmology and Visual Neurosciences, University of Minnesota, Minneapolis, Minnesota, United States of America., Karunadharma PP; Department of Ophthalmology and Visual Neurosciences, University of Minnesota, Minneapolis, Minnesota, United States of America., Terluk MR; Department of Ophthalmology and Visual Neurosciences, University of Minnesota, Minneapolis, Minnesota, United States of America., Kapphahn RJ; Department of Ophthalmology and Visual Neurosciences, University of Minnesota, Minneapolis, Minnesota, United States of America., Olsen TW; Department of Ophthalmology and Visual Neurosciences, University of Minnesota, Minneapolis, Minnesota, United States of America.; Department of Ophthalmology, Mayo Clinic, Rochester, Minnesota, United States of America., Ferrington DA; Department of Ophthalmology and Visual Neurosciences, University of Minnesota, Minneapolis, Minnesota, United States of America., Montezuma SR; Department of Ophthalmology and Visual Neurosciences, University of Minnesota, Minneapolis, Minnesota, United States of America.
Jazyk: angličtina
Zdroj: PloS one [PLoS One] 2021 Oct 19; Vol. 16 (10), pp. e0258803. Date of Electronic Publication: 2021 Oct 19 (Print Publication: 2021).
DOI: 10.1371/journal.pone.0258803
Abstrakt: Purpose: To determine whether age-related macular degeneration (AMD) severity or the frequency of retinal pigment epithelium mitochondrial DNA lesions differ in human donor eyes that have undergone cataract surgery compared to phakic eyes.
Methods: Eyes from human donors aged ≥ 55 years were obtained from the Minnesota Lions Eye Bank. Cataract surgery status was obtained from history provided to Eye Bank personnel by family members at the time of tissue procurement. Donor eyes were graded for AMD severity using the Minnesota Grading System. Quantitative PCR was performed on DNA isolated from macular punches of retinal pigment epithelium to quantitate the frequency of mitochondrial DNA lesions in the donor tissue. Univariable and multivariable analyses were performed to evaluate for associations between (1) cataract surgery and AMD severity and (2) cataract surgery and mitochondrial DNA lesion frequency.
Results: A total of 157 subjects qualified for study inclusion. Multivariable analysis with age, sex, smoking status, and cataract surgery status showed that only age was associated with AMD grade. Multivariable analysis with age, sex, smoking status, and cataract surgery status showed that none of these factors were associated with retinal pigment epithelium mitochondrial DNA lesion frequency.
Conclusions: In this study of human donor eyes, neither retinal pigment epithelium mitochondrial DNA damage nor the stage of AMD severity are independently associated with cataract surgery after adjusting for other AMD risk factors. These new pathologic and molecular findings provide evidence against a relationship between cataract surgery and AMD progression and support the idea that cataract surgery is safe in the setting of AMD.
Competing Interests: I have read the journal’s policy and the authors of this manuscript have the following competing interests: TWO: Equity owner – iMacular Regeneration LLC. DAF: Scientific advisory board member – Vinci Pharmaceuticals. The authors have no proprietary or commercial interest in any materials discussed in this article. This does not alter our adherence to PLOS ONE policies on sharing data and materials.
Databáze: MEDLINE
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