Electrochemical characterization of mutant forms of rubredoxin B from Mycobacterium tuberculosis.

Autor: Gilep A; Institute of Bioorganic Chemistry, National Academy of Sciences of Belarus, Minsk, Belarus; Institute of Biomedical Chemistry, Moscow, Russia., Kuzikov A; Institute of Biomedical Chemistry, Moscow, Russia; Pirogov Russian National Research Medical University, Moscow, Russia., Sushko T; IMSUT, The University of Tokyo, Tokyo, Japan., Grabovec I; Institute of Bioorganic Chemistry, National Academy of Sciences of Belarus, Minsk, Belarus., Masamrekh R; Institute of Biomedical Chemistry, Moscow, Russia; Pirogov Russian National Research Medical University, Moscow, Russia., Sigolaeva LV; Department of Chemistry, M.V. Lomonosov Moscow State University, 119991 Moscow, Russia., Pergushov DV; Department of Chemistry, M.V. Lomonosov Moscow State University, 119991 Moscow, Russia., Schacher FH; Institute of Organic Chemistry and Macromolecular Chemistry (IOMC), Friedrich-Schiller-University Jena, D-07743 Jena, Germany; Jena Center for Soft Matter (JCSM), Friedrich-Schiller-University Jena, D-07743 Jena, Germany; Center for Energy and Environmental Chemistry (CEEC), Friedrich-Schiller-University Jena, D-07743 Jena, Germany., Strushkevich N; Skolkovo Institute of Science and Technology, Moscow, Russia. Electronic address: n.strushkevich@skoltech.ru., Shumyantseva VV; Institute of Biomedical Chemistry, Moscow, Russia; Pirogov Russian National Research Medical University, Moscow, Russia. Electronic address: viktoria.shumyantseva@ibmc.msk.ru.
Jazyk: angličtina
Zdroj: Biochimica et biophysica acta. Proteins and proteomics [Biochim Biophys Acta Proteins Proteom] 2022 Jan; Vol. 1870 (1), pp. 140734. Date of Electronic Publication: 2021 Oct 16.
DOI: 10.1016/j.bbapap.2021.140734
Abstrakt: Electron transfer in metalloproteins is a driving force for many biological processes and widely distributed in nature. Rubredoxin B (RubB) from Mycobacterium tuberculosis is a first example among [1Fe-0S] proteins that support catalytic activity of terminal sterol-monooxygenases enabling its application in metabolic engineering. To explore the tolerance of RubB to the specific amino acid changes we evaluated the effect of surface mutations on its electrochemical properties. Based on the RubB fold we also designed the mutant with a putative additional site for protein-protein interactions to further evaluate electron transfer and electrochemical properties. The investigation of redox properties of mutant variants of RubB was done using screen-printed graphite electrodes (SPEs) modified with stable dispersion of multi-walled carbon nanotubes (MWCNTs). The redox potentials (midpoint potentials, E 0Ꞌ ) of mutants did not significantly differ from the wild type protein and vary in the range of -264 to -231 mV vs. Ag/AgCl electrode. However, all mutations affect electron transfer rate between the protein and electrode. Notably, the modulation of the protein-protein interactions was observed for the insertion mutant suggesting the possibility of tailoring of rubredoxin for the selected redox-partner. Overall, RubB is tolerant to the significant modifications in its structure enabling rational engineering of novel redox proteins.
(Copyright © 2021. Published by Elsevier B.V.)
Databáze: MEDLINE
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