Cattle and goats' humoral response to vaccination with Clostridium perfringens type D purified epsilon toxoids.

Autor: Oliveira RC; Veterinary School, Universidade Federal de Minas Gerais (UFMG), Avenida Antônio Carlos, 6627, Belo Horizonte, CEP 31.270-901, MG, Brazil; Merck Sharp & Dohme (MSD), Avenida Comendador Loureiro Ramos, 1500, Montes Claros, CEP 39.404-003, MG, Brazil., de Oliveira Júnior CA; Veterinary School, Universidade Federal de Minas Gerais (UFMG), Avenida Antônio Carlos, 6627, Belo Horizonte, CEP 31.270-901, MG, Brazil., Alves GG; Veterinary School, Universidade Federal de Minas Gerais (UFMG), Avenida Antônio Carlos, 6627, Belo Horizonte, CEP 31.270-901, MG, Brazil., Assis RA; Veterinary School, Universidade Federal de Minas Gerais (UFMG), Avenida Antônio Carlos, 6627, Belo Horizonte, CEP 31.270-901, MG, Brazil; Merck Sharp & Dohme (MSD), Avenida Comendador Loureiro Ramos, 1500, Montes Claros, CEP 39.404-003, MG, Brazil., Silva ROS; Veterinary School, Universidade Federal de Minas Gerais (UFMG), Avenida Antônio Carlos, 6627, Belo Horizonte, CEP 31.270-901, MG, Brazil., de Sousa Xavier MA; Universidade Estadual de Montes Claros (UNIMONTES), Avenida, Professor Rui Braga, S/N Montes Claros, CEP 39.401-089, MG, Brazil., Lobato FCF; Veterinary School, Universidade Federal de Minas Gerais (UFMG), Avenida Antônio Carlos, 6627, Belo Horizonte, CEP 31.270-901, MG, Brazil. Electronic address: lobato.francisco@yahoo.com.br.
Jazyk: angličtina
Zdroj: Anaerobe [Anaerobe] 2021 Dec; Vol. 72, pp. 102465. Date of Electronic Publication: 2021 Oct 16.
DOI: 10.1016/j.anaerobe.2021.102465
Abstrakt: Herd vaccination is an important preventive measure against enterotoxemia in ruminants. Vaccination in goats should be performed every four months, and recent studies have shown that immunity in cattle lasts for less than one year. One of the mechanisms for increasing the duration of the immune response is to use purified toxoids as immunogens. The aim of the present study was to evaluate the humoral response in cattle and goats after vaccination with purified and semi-purified Clostridium perfringens type D epsilon toxoid. The following three different vaccines were used: vaccine 1 (V1), a semi-purified toxoid adsorbed to aluminum hydroxide; vaccine 2 (V2), a purified toxoid adsorbed to aluminum hydroxide; and vaccine (V3), a purified toxoid adsorbed on chitosan microparticles. Groups of cattle (n = 6-7) and goats (n = 6-7) were vaccinated on days 0 and 30, and serum samples for antitoxin titration were collected every 30 days for one-year post-vaccination. Goats were revaccinated on day 360, and their serum was evaluated on days 367 and 374. The antibody peaks ranged between 6.90 and 11.47 IU/mL in cattle and from 1.11 to 4.40 IU/mL in goats. In cattle administered with the V1 and V2 vaccines, we observed that the antibody titers were maintained above 0.2 IU/mL until the end of the experiment. In goats, V2 elicited long-lasting antibodies, and all animals maintained the protective titers for 210 days after the first dose. In conclusion, the purified toxoid vaccine with aluminum hydroxide adjuvant was able to induce strong and long-lasting humoral responses in both species and could be an alternative for improving the immunization schedule against enterotoxemia in goats and cattle.
Competing Interests: Declaration of competing interest The authors declare that there are no conflicts of interest.
(Copyright © 2021 Elsevier Ltd. All rights reserved.)
Databáze: MEDLINE
Externí odkaz: