In vivo sensing of cutaneous edema: A comparative study of diffuse reflectance, Raman spectroscopy and multispectral imaging.

Autor: Budylin GS; Medical Research and Education Center, M. V. Lomonosov Moscow State University, Moscow, Russia.; Institute of Spectroscopy of the Russian Academy of Sciences, Moscow, Russia.; World-Class Research Center 'Digital Biodesign and Personalized Healthcare', Sechenov First Moscow State Medical University, Moscow, Russia., Davydov DA; Medical Research and Education Center, M. V. Lomonosov Moscow State University, Moscow, Russia.; Faculty of Physics, Lomonosov Moscow State University, Moscow, Russia., Zlobina NV; Medical Research and Education Center, M. V. Lomonosov Moscow State University, Moscow, Russia.; Faculty of Physics, Lomonosov Moscow State University, Moscow, Russia., Baev AV; Faculty of Physics, Lomonosov Moscow State University, Moscow, Russia., Artyushenko VG; Art Photonics GmbH, Berlin, Germany., Yakimov BP; Medical Research and Education Center, M. V. Lomonosov Moscow State University, Moscow, Russia.; World-Class Research Center 'Digital Biodesign and Personalized Healthcare', Sechenov First Moscow State Medical University, Moscow, Russia.; Faculty of Physics, Lomonosov Moscow State University, Moscow, Russia., Shirshin EA; Medical Research and Education Center, M. V. Lomonosov Moscow State University, Moscow, Russia.; World-Class Research Center 'Digital Biodesign and Personalized Healthcare', Sechenov First Moscow State Medical University, Moscow, Russia.; Faculty of Physics, Lomonosov Moscow State University, Moscow, Russia.
Jazyk: angličtina
Zdroj: Journal of biophotonics [J Biophotonics] 2022 Jan; Vol. 15 (1), pp. e202100268. Date of Electronic Publication: 2021 Oct 30.
DOI: 10.1002/jbio.202100268
Abstrakt: Quantitative noninvasive assessment of water content in tissues is important for biomedicine. Optical spectroscopy is potentially capable of solving this problem; however, its applicability for clinical diagnostics remains questionable. The presented study compares diffuse reflectance spectroscopy, Raman spectroscopy and multispectral imaging in the characterization of cutaneous edema. The source-detector geometries for each method are selected based on Monte Carlo simulations results to detect the signal from the dermis. Then, the kinetics of the edema development is studied for two models. All methods demonstrate synchronous trends for histamine-induced edema: The water content reaches a maximum of 1 hour after histamine application and then gradually decreases. For the venous occlusion, a 51% increase in water content is observed with Raman spectroscopy. The differences in water content estimation by three methods are explained based on the light propagation model. The obtained results are essential for introducing quantitative optical water measurement technology to the clinics.
(© 2021 Wiley-VCH GmbH.)
Databáze: MEDLINE
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