Characterization and genomic analysis of Exiguobacterium alkaliphilum B-3531D, an efficient crude oil degrading strain.

Autor:	Delegan Y; Skryabin Institute of Biochemistry and Physiology of Microorganisms, Pushchino Scientific Center for Biological Research of Russian Academy of Sciences, Pushchino, Moscow Oblast, 142290, Russian Federation., Kocharovskaya Y; Skryabin Institute of Biochemistry and Physiology of Microorganisms, Pushchino Scientific Center for Biological Research of Russian Academy of Sciences, Pushchino, Moscow Oblast, 142290, Russian Federation.; Pushchino State Institute of Natural Science, Pushchino, Moscow Oblast, 142290, Russian Federation., Bogun A; State Research Center for Applied Microbiology and Biotechnology, Obolensk, 142279, Russian Federation., Sizova A; State Research Center for Applied Microbiology and Biotechnology, Obolensk, 142279, Russian Federation., Solomentsev V; State Research Center for Applied Microbiology and Biotechnology, Obolensk, 142279, Russian Federation., Iminova L; Pushchino State Institute of Natural Science, Pushchino, Moscow Oblast, 142290, Russian Federation., Lyakhovchenko N; Belgorod State University, Belgorod, 308015, Russian Federation., Zinovieva A; Belgorod State University, Belgorod, 308015, Russian Federation., Goyanov M; Belgorod State University, Belgorod, 308015, Russian Federation., Solyanikova I; Skryabin Institute of Biochemistry and Physiology of Microorganisms, Pushchino Scientific Center for Biological Research of Russian Academy of Sciences, Pushchino, Moscow Oblast, 142290, Russian Federation.; Belgorod State University, Belgorod, 308015, Russian Federation.
Jazyk:	angličtina
Zdroj:	Biotechnology reports (Amsterdam, Netherlands) [Biotechnol Rep (Amst)] 2021 Sep 28; Vol. 32, pp. e00678. Date of Electronic Publication: 2021 Sep 28 (Print Publication: 2021).
DOI:	10.1016/j.btre.2021.e00678
Abstrakt:	The aim of the work was to carry out the physiological, biochemical and genetic characterization of the Exiguobacterium alkaliphilum B-3531D strain. This strain is promising for use in the field of environmental biotechnology, since it has a pronounced ability to utilize crude oil and individual hydrocarbons in a wide temperature range. The genome of the strain was sequenced and completely assembled, it consists of a 2,903,369 bp circular chromosome and two circular plasmids, namely, pE73 (73,590 bp) and pE52 (52,125 bp). When cultivated in a mineral medium containing 2% of crude oil, the strain utilized 50% within 30 days of the experiment. In simulated seawater with the same oil content, the loss of hydrocarbons was 45% over the same period. For the first time we observed in an E. alkaliphilum strain the ability to efficiently utilize crude oil, including with an increased content of sodium chloride in the cultivation medium. Competing Interests: Let me inform you that all authors (Yanina Delegan, Yulia Kocharovskaya, Alexander Bogun, Angelika Sizova, Viktor Solomentsev, Leila Iminova, Nikita Lyakhovchenko, Alina Zinovieva, Mikhail Goyanov, Inna Solyanikova) declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. (© 2021 The Authors. Published by Elsevier B.V.)
Databáze:	MEDLINE
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