RP-HPLC-DAD determination of the differences in the polyphenol content of Fucus vesiculosus extracts with similar antioxidant activity.

Autor: Sánchez-Bonet D; Escuela de Doctorado, Universidad Católica de Valencia 'San Vicente Mártir', C/Guillem de Castro, 65, 46008 Valencia, Spain., García-Oms S; Escuela de Doctorado, Universidad Católica de Valencia 'San Vicente Mártir', C/Guillem de Castro, 65, 46008 Valencia, Spain., Belda-Antolí M; Centro de Investigación Traslacional San Alberto Magno, Universidad Católica de Valencia 'San Vicente Mártir', C/Quevedo, 2, 46001 Valencia, Spain., Padrón-Sanz C; Centro de Investigación Traslacional San Alberto Magno, Universidad Católica de Valencia 'San Vicente Mártir', C/Quevedo, 2, 46001 Valencia, Spain., Lloris-Carsi JM; Facultad de Medicina, Departamento de Cirugía. Universidad de Valencia, Avda. Blasco Ibáñez, 13, 46010 Valencia, Spain., Cejalvo-Lapeña D; Centro de Investigación Traslacional San Alberto Magno, Universidad Católica de Valencia 'San Vicente Mártir', C/Quevedo, 2, 46001 Valencia, Spain.
Jazyk: angličtina
Zdroj: Journal of chromatography. B, Analytical technologies in the biomedical and life sciences [J Chromatogr B Analyt Technol Biomed Life Sci] 2021 Nov 01; Vol. 1184, pp. 122978. Date of Electronic Publication: 2021 Oct 05.
DOI: 10.1016/j.jchromb.2021.122978
Abstrakt: Significant quantities of bioactive compounds have been found in the chemical composition of seaweeds. This source of natural antioxidants such as polyphenols appears to attenuate lipid peroxidation caused by oxidative stress, preventing the harmful effects of a number of injuries including ischemia-reperfusion (I/R). Conventional extraction (CE) has been used for years as a traditional method for obtaining bioactive components from seaweeds. However, recent studies highlight ultrasonic-assisted extraction (UAE) as an alternative and more eco-friendly technique. Therefore, the two methods were optimised and compared to obtain a Fucus vesiculosus extract (FVE) with high antioxidant activity and polyphenol content. The highest antioxidant activity was obtained after 1 h at 25 °C for conventional extraction, and after 5 min at 35 °C for ultrasonic-assisted extraction. Higher concentrations of polyphenols were obtained with the optimal conditions in conventional extraction (13.61 mg PGE/g seaweed), but no significant differences were observed between the antioxidant activity obtained with UAE (89.33%) and CE (89.74%). The characterization of the polyphenols present in both optimised extracts was carried out and compared with reverse-phase high-performance liquid chromatography coupled to a diode array detector (HPLC-DAD). The following compounds were identified: phloroglucinol, gallic acid, catechin, vanillic acid, epicatechin, protocatechuic acid, rutin, gentisic acid, chlorogenic acid, caffeic acid, coumaric acid and ferulic acid. RP-HPLC-DAD results also showed higher concentrations of polyphenols in optimised extracts with CE. Consequently, CE was found to be more effective than UAE in providing extracts with higher concentrations of polyphenols, but UAE constitutes an efficient and more eco-friendly methodology for obtaining a FVE with the highest antioxidant activity.
(Copyright © 2021 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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