Encapsulation and dispersion of Lactobacillus acidophilus in a chocolate coating as a strategy for maintaining cell viability in cereal bars.

Autor: Lasta EL; Programa de Pós-Graduação em Tecnologia de Processos Químicos e Bioquímicos, Universidade Tecnológica Federal do Paraná, Via do Conhecimento Km 01, Pato Branco, Paraná, CEP 85503-390, Brazil., da Silva Pereira Ronning E; Departamento de Química, Universidade Tecnológica Federal do Paraná, Via do Conhecimento Km 01, Pato Branco, Paraná, CEP 85503-390, Brazil.; Grupo de Pesquisa em Tecnologia de Bioprocessos e Alimentos (GTBio), Universidade Tecnológica Federal do Paraná, Via do Conhecimento Km 01, Pato Branco, Paraná, CEP 85503-390, Brazil., Dekker RFH; Grupo de Pesquisa em Tecnologia de Bioprocessos e Alimentos (GTBio), Universidade Tecnológica Federal do Paraná, Via do Conhecimento Km 01, Pato Branco, Paraná, CEP 85503-390, Brazil.; Beta-Glucan Produtos Farmoquímicos EIRELI, Lote 24A, Bloco Zircônia, Universidade Tecnológica Federal do Paraná, Câmpus Londrina, Avenida João Miguel Caram 731, Londrina, Paraná, CEP 86036-700, Brazil., da Cunha MAA; Departamento de Química, Universidade Tecnológica Federal do Paraná, Via do Conhecimento Km 01, Pato Branco, Paraná, CEP 85503-390, Brazil. mcunha@utfpr.edu.br.; Grupo de Pesquisa em Tecnologia de Bioprocessos e Alimentos (GTBio), Universidade Tecnológica Federal do Paraná, Via do Conhecimento Km 01, Pato Branco, Paraná, CEP 85503-390, Brazil. mcunha@utfpr.edu.br.
Jazyk: angličtina
Zdroj: Scientific reports [Sci Rep] 2021 Oct 15; Vol. 11 (1), pp. 20550. Date of Electronic Publication: 2021 Oct 15.
DOI: 10.1038/s41598-021-00077-0
Abstrakt: Flour from Pereskia aculeata leaf and green banana were used as ingredients in the formulation of a cereal bar with added Lactobacillus acidophilus LA02-ID-1688. Encapsulation in a calcium-alginate hydrogel reinforced with magnesium hydroxide was used as a strategy to protect the probiotic cells under gastrointestinal conditions and to prolong shelf-life. The results are relevant especially for maintaining cell viability during shelf-life; a challenge for the food industry in relation to dry probiotic products. Encapsulation promoted the protection of probiotic cells in simulated gastric and intestinal conditions, allowing the maintenance of high viable cell counts (> 10 log CFU, colony forming unit). Encapsulation also contributed to cellular protection under extreme temperature conditions, with reductions of cell viability of < 1 logarithmic cycle when the capsules were subjected to 55ºC/10 min. Even at 75ºC/10 min, encapsulation protected the probiotic cells 3-times greater than the free-cells. The food bar proved to be rich in dietary fiber (19 g 100 g -1 ), lipids (12.63 g 100 g -1 ) and showed an appreciable protein content (5.44 g 100 g -1 ). A high viable probiotic cell count on storage over 120 days (12.54 log CFU) was observed, maintaining a probiotic survival rate > 90% and viability levels sufficient to promote health benefits.
(© 2021. The Author(s).)
Databáze: MEDLINE
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