Compensatory ion transport buffers daily protein rhythms to regulate osmotic balance and cellular physiology.

Autor: Stangherlin A; MRC Laboratory of Molecular Biology, Cambridge, UK., Watson JL; MRC Laboratory of Molecular Biology, Cambridge, UK., Wong DCS; MRC Laboratory of Molecular Biology, Cambridge, UK., Barbiero S; MRC Laboratory of Molecular Biology, Cambridge, UK., Zeng A; MRC Laboratory of Molecular Biology, Cambridge, UK., Seinkmane E; MRC Laboratory of Molecular Biology, Cambridge, UK., Chew SP; MRC Laboratory of Molecular Biology, Cambridge, UK., Beale AD; MRC Laboratory of Molecular Biology, Cambridge, UK., Hayter EA; Faculty of Biology, Medicine and Health, University of Manchester, Manchester, UK., Guna A; UCSF, San Francisco, CA, USA., Inglis AJ; California Institute of Technology, Pasadena, CA, USA., Putker M; MRC Laboratory of Molecular Biology, Cambridge, UK.; Crown Bioscience Netherlands B.V., Utrecht, The Netherlands., Bartolami E; Department of Chemistry, University of Geneva, Geneva, Switzerland.; CEA, IRIG, SyMMES, Grenoble, France., Matile S; Department of Chemistry, University of Geneva, Geneva, Switzerland., Lequeux N; LPEM - ESPCI Paris, PSL, CNRS, Sorbonne Université, Paris, France., Pons T; LPEM - ESPCI Paris, PSL, CNRS, Sorbonne Université, Paris, France., Day J; Department of Earth Sciences, University of Cambridge, Cambridge, UK., van Ooijen G; School of Biological Sciences, University of Edinburgh, Edinburgh, UK., Voorhees RM; California Institute of Technology, Pasadena, CA, USA., Bechtold DA; Faculty of Biology, Medicine and Health, University of Manchester, Manchester, UK., Derivery E; MRC Laboratory of Molecular Biology, Cambridge, UK., Edgar RS; Department of Infectious Diseases, Imperial College London, London, UK., Newham P; Clinical Pharmacology and Safety Sciences, R&D, AstraZeneca, Cambridge, UK., O'Neill JS; MRC Laboratory of Molecular Biology, Cambridge, UK. oneillj@mrc-lmb.cam.ac.uk.
Jazyk: angličtina
Zdroj: Nature communications [Nat Commun] 2021 Oct 15; Vol. 12 (1), pp. 6035. Date of Electronic Publication: 2021 Oct 15.
DOI: 10.1038/s41467-021-25942-4
Abstrakt: Between 6-20% of the cellular proteome is under circadian control and tunes mammalian cell function with daily environmental cycles. For cell viability, and to maintain volume within narrow limits, the daily variation in osmotic potential exerted by changes in the soluble proteome must be counterbalanced. The mechanisms and consequences of this osmotic compensation have not been investigated before. In cultured cells and in tissue we find that compensation involves electroneutral active transport of Na + , K + , and Cl - through differential activity of SLC12A family cotransporters. In cardiomyocytes ex vivo and in vivo, compensatory ion fluxes confer daily variation in electrical activity. Perturbation of soluble protein abundance has commensurate effects on ion composition and cellular function across the circadian cycle. Thus, circadian regulation of the proteome impacts ion homeostasis with substantial consequences for the physiology of electrically active cells such as cardiomyocytes.
(© 2021. Crown.)
Databáze: MEDLINE
Externí odkaz: