Direct on-swab metabolic profiling of vaginal microbiome host interactions during pregnancy and preterm birth.

Autor:	Pruski P; Division of Systems Medicine, Department of Metabolism, Digestion and Reproduction, Faculty of Medicine Imperial College London, London, UK., Correia GDS; Division of Systems Medicine, Department of Metabolism, Digestion and Reproduction, Faculty of Medicine Imperial College London, London, UK.; National Phenome Centre, Imperial College London, London, UK., Lewis HV; March of Dimes Prematurity Research Centre at Imperial College London, London, UK.; Imperial College Parturition Research Group, Institute of Reproductive and Developmental Biology, Department of Metabolism, Digestion and Reproduction, Imperial College London, London, UK.; Queen Charlotte's & Chelsea Hospital, Imperial College London, London, UK., Capuccini K; March of Dimes Prematurity Research Centre at Imperial College London, London, UK.; Imperial College Parturition Research Group, Institute of Reproductive and Developmental Biology, Department of Metabolism, Digestion and Reproduction, Imperial College London, London, UK., Inglese P; Division of Systems Medicine, Department of Metabolism, Digestion and Reproduction, Faculty of Medicine Imperial College London, London, UK.; National Phenome Centre, Imperial College London, London, UK., Chan D; March of Dimes Prematurity Research Centre at Imperial College London, London, UK.; Imperial College Parturition Research Group, Institute of Reproductive and Developmental Biology, Department of Metabolism, Digestion and Reproduction, Imperial College London, London, UK.; Queen Charlotte's & Chelsea Hospital, Imperial College London, London, UK., Brown RG; Imperial College Parturition Research Group, Institute of Reproductive and Developmental Biology, Department of Metabolism, Digestion and Reproduction, Imperial College London, London, UK.; Queen Charlotte's & Chelsea Hospital, Imperial College London, London, UK., Kindinger L; Imperial College Parturition Research Group, Institute of Reproductive and Developmental Biology, Department of Metabolism, Digestion and Reproduction, Imperial College London, London, UK.; Elizabeth Garrett Anderson Institute for Women's Health, University College London, London, UK., Lee YS; March of Dimes Prematurity Research Centre at Imperial College London, London, UK.; Imperial College Parturition Research Group, Institute of Reproductive and Developmental Biology, Department of Metabolism, Digestion and Reproduction, Imperial College London, London, UK., Smith A; Faculty of Health and Applied Sciences, University West of England, Bristol, UK., Marchesi J; Division of Systems Medicine, Department of Metabolism, Digestion and Reproduction, Faculty of Medicine Imperial College London, London, UK.; March of Dimes Prematurity Research Centre at Imperial College London, London, UK., McDonald JAK; MRC Centre for Molecular Bacteriology and Infection, Imperial College London, London, UK., Cameron S; Division of Systems Medicine, Department of Metabolism, Digestion and Reproduction, Faculty of Medicine Imperial College London, London, UK.; School of Biological Sciences, Institute for Global Food Security, Queen's University Belfast, Belfast, UK., Alexander-Hardiman K; Division of Systems Medicine, Department of Metabolism, Digestion and Reproduction, Faculty of Medicine Imperial College London, London, UK., David AL; Elizabeth Garrett Anderson Institute for Women's Health, University College London, London, UK., Stock SJ; MRC Centre for Reproductive Health, University of Edinburgh, Edinburgh, UK., Norman JE; MRC Centre for Reproductive Health, University of Edinburgh, Edinburgh, UK.; Faculty of Health Sciences, University of Bristol, Bristol, UK., Terzidou V; March of Dimes Prematurity Research Centre at Imperial College London, London, UK.; Imperial College Parturition Research Group, Institute of Reproductive and Developmental Biology, Department of Metabolism, Digestion and Reproduction, Imperial College London, London, UK.; Chelsea & Westminster Hospital, NHS Trust, London, UK., Teoh TG; St Mary's Hospital, Imperial College Healthcare NHS Trust, London, UK., Sykes L; March of Dimes Prematurity Research Centre at Imperial College London, London, UK.; Imperial College Parturition Research Group, Institute of Reproductive and Developmental Biology, Department of Metabolism, Digestion and Reproduction, Imperial College London, London, UK.; Queen Charlotte's & Chelsea Hospital, Imperial College London, London, UK., Bennett PR; March of Dimes Prematurity Research Centre at Imperial College London, London, UK.; Imperial College Parturition Research Group, Institute of Reproductive and Developmental Biology, Department of Metabolism, Digestion and Reproduction, Imperial College London, London, UK.; Queen Charlotte's & Chelsea Hospital, Imperial College London, London, UK.; Tommy's National Centre for Miscarriage Research, Imperial College London, London, UK., Takats Z; Division of Systems Medicine, Department of Metabolism, Digestion and Reproduction, Faculty of Medicine Imperial College London, London, UK. z.takats@imperial.ac.uk.; National Phenome Centre, Imperial College London, London, UK. z.takats@imperial.ac.uk.; March of Dimes Prematurity Research Centre at Imperial College London, London, UK. z.takats@imperial.ac.uk., MacIntyre DA; March of Dimes Prematurity Research Centre at Imperial College London, London, UK. d.macintyre@imperial.ac.uk.; Imperial College Parturition Research Group, Institute of Reproductive and Developmental Biology, Department of Metabolism, Digestion and Reproduction, Imperial College London, London, UK. d.macintyre@imperial.ac.uk.; Tommy's National Centre for Miscarriage Research, Imperial College London, London, UK. d.macintyre@imperial.ac.uk.
Jazyk:	angličtina
Zdroj:	Nature communications [Nat Commun] 2021 Oct 13; Vol. 12 (1), pp. 5967. Date of Electronic Publication: 2021 Oct 13.
DOI:	10.1038/s41467-021-26215-w
Abstrakt:	The pregnancy vaginal microbiome contributes to risk of preterm birth, the primary cause of death in children under 5 years of age. Here we describe direct on-swab metabolic profiling by Desorption Electrospray Ionization Mass Spectrometry (DESI-MS) for sample preparation-free characterisation of the cervicovaginal metabolome in two independent pregnancy cohorts (VMET, n = 160; 455 swabs; VMET II, n = 205; 573 swabs). By integrating metataxonomics and immune profiling data from matched samples, we show that specific metabolome signatures can be used to robustly predict simultaneously both the composition of the vaginal microbiome and host inflammatory status. In these patients, vaginal microbiota instability and innate immune activation, as predicted using DESI-MS, associated with preterm birth, including in women receiving cervical cerclage for preterm birth prevention. These findings highlight direct on-swab metabolic profiling by DESI-MS as an innovative approach for preterm birth risk stratification through rapid assessment of vaginal microbiota-host dynamics. (© 2021. The Author(s).)
Databáze:	MEDLINE
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