Identification of active deubiquitinases in the chicken tissues.

Autor: Nanduri B; Department of Comparative Biomedical Sciences, College of Veterinary Medicine, Mississippi State University, Mississippi State, Mississippi, USA.; Institute for Genomics, Biocomputing, and Biotechnology, Mississippi State University, Mississippi State, Mississippi, USA., Gresham CR; Institute for Genomics, Biocomputing, and Biotechnology, Mississippi State University, Mississippi State, Mississippi, USA., Jones G; Department of Microbiology and Cell Science, College of Agricultural and Life Sciences, University of Florida, Gainesville, USA., Bailey RH; Department of Pathobiology and Population Medicine, College of Veterinary Medicine, Mississippi State University, Mississippi State, Mississippi, USA., Edelmann MJ; Department of Microbiology and Cell Science, College of Agricultural and Life Sciences, University of Florida, Gainesville, USA.
Jazyk: angličtina
Zdroj: Proteomics [Proteomics] 2022 Jan; Vol. 22 (1-2), pp. e2100122. Date of Electronic Publication: 2021 Oct 23.
DOI: 10.1002/pmic.202100122
Abstrakt: The existing protein annotation in chicken is mostly limited to computational predictions based on orthology to other proteins, which often leads to a significant underestimation of the function of these proteins. Genome-scale experimental annotation can provide insight into the actual enzymatic activities of chicken proteins. Amongst post-translational modifications, ubiquitination is of interest as anomalies in ubiquitination are implicated in such diseases as inflammatory disorders, infectious diseases, or malignancies. Ubiquitination is controlled by deubiquitinases (DUBs), which remove ubiquitin from protein substrates. However, the DUBs have not been systematically annotated and quantified in chicken tissues. Here we used a chemoproteomics approach, which is based on active-site probes specific to DUBs, and identified 26 active DUBs in the chicken spleen, cecum, and liver.
(© 2021 Wiley-VCH GmbH.)
Databáze: MEDLINE
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