Subproteomic profiling from renal cortices in OLETF rats reveals mutations of multiple novel genes in diabetic nephropathy.

Autor: Li Z; Department of School of Public Health, International Science and Technology Cooperation Base of Geriatric Medicine, The Hebei Key Lab for Organ Fibrosis, The Hebei Key Lab for chronic disease, North China University of Science and Technology, Tangshan, 063000, China., Shen H; Department of Modern Technology and Education, North China University of Science and Technology, Tangshan, 063000, China., Liu Y; Department of Endocrinology, Kailuan General Hospital, North China University of Science and Technology, Tangshan, 063000, China., Zhou X; Beijing Key Lab for Immune-Mediated Inflammatory Diseases, Institute of Clinical Medical Science, China-Japan Friendship Hospital, 2 Yinghua East Road, Chaoyang District, Beijing, 100029, People's Republic of China., Yan M; Beijing Key Lab for Immune-Mediated Inflammatory Diseases, Institute of Clinical Medical Science, China-Japan Friendship Hospital, 2 Yinghua East Road, Chaoyang District, Beijing, 100029, People's Republic of China., He H; School of Graduate Studies, North China University of Science and Technology, Tangshan, 063000, China., Zhao T; Beijing Key Lab for Immune-Mediated Inflammatory Diseases, Institute of Clinical Medical Science, China-Japan Friendship Hospital, 2 Yinghua East Road, Chaoyang District, Beijing, 100029, People's Republic of China., Zhang H; Beijing Key Lab for Immune-Mediated Inflammatory Diseases, Institute of Clinical Medical Science, China-Japan Friendship Hospital, 2 Yinghua East Road, Chaoyang District, Beijing, 100029, People's Republic of China. zhhj529@163.com., Li P; Beijing Key Lab for Immune-Mediated Inflammatory Diseases, Institute of Clinical Medical Science, China-Japan Friendship Hospital, 2 Yinghua East Road, Chaoyang District, Beijing, 100029, People's Republic of China. lp8675@163.com.
Jazyk: angličtina
Zdroj: Genes & genomics [Genes Genomics] 2022 Jan; Vol. 44 (1), pp. 109-122. Date of Electronic Publication: 2021 Oct 13.
DOI: 10.1007/s13258-021-01174-0
Abstrakt: Background: Diabetic nephropathy (DN) is a serious threat to human health, but its pathogenesis is not fully understood. Otsuka Long-Evans Tokushima Fatty (OLETF) rats are very similar to human DN in many aspects such as pathological changes and processes, and are deemed to be an ideal rodent model.
Objective: This study was aimed to explore the pathogenesis of DN by analyzing the protein expression profile from renal cortices in OLETF rats.
Methods: Thirty-six-week-old diabetic OLETF rats and normal control Long-Evans Tokushima Otsuka (LETO) rats were nephrectomized, and the renal cortices were isolated. The proteins were separated by soluble and insoluble high-resolution subproteomics methods for the analysis and identification of differential proteins.
Results: Thirty-six differentially expressed proteins were found. Among them, 11 proteins had different isoelectric points and molecular weights between OLETF and LETO rats. Further sequencing identified point mutations in genes encoding eight of these proteins, which are involved in many biological processes closely related to DN, including oxidative stress and inflammation. Five of these eight proteins have not been reported in DN.
Conclusion: This study reveals mutations of multiple novel genes in diabetic OLETF rats, providing some new potential targets for the pathogenesis of DN and helping to better understand the pathogenesis of DN.
(© 2021. The Genetics Society of Korea.)
Databáze: MEDLINE
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