Low-Density Granulocytes and Neutrophil Extracellular Traps as Biomarkers of Disease Activity in Adult Inflammatory Myopathies.

Autor:	Torres-Ruiz J, Carrillo-Vázquez DA; From the Departments of Immunology and Rheumatology., Leal-Alanis A; Internal Medicine, Instituto Nacional de Ciencias Medicas y Nutricion Salvador Zubiran., Zentella-Dehesa A; Department of Genomic Medicine and Environmental Toxicology., Tapia-Rodríguez M; Microscopy Unit, Instituto de Investigaciones Biomedicas, Universidad Nacional Autonoma de Mexico., Maravillas-Montero JL; Metabolomic and Proteomic Unit., Nuñez-Álvarez CA; From the Departments of Immunology and Rheumatology., Carazo-Vargas ER; From the Departments of Immunology and Rheumatology., Romero-Hernández I; From the Departments of Immunology and Rheumatology., Juárez-Vega G; Flow Cytometry Unit, Red de Apoyo a la Investigación, Coordinacion de Investigación Cientifica, Universidad Nacional Autonoma de Mexico, Mexico City, Mexico., Alcocer-Varela J; From the Departments of Immunology and Rheumatology., Gómez-Martín D
Jazyk:	angličtina
Zdroj:	Journal of clinical rheumatology : practical reports on rheumatic & musculoskeletal diseases [J Clin Rheumatol] 2022 Mar 01; Vol. 28 (2), pp. e480-e487.
DOI:	10.1097/RHU.0000000000001772
Abstrakt:	Background/objective: Biomarkers for disease activity and damage accrual in idiopathic inflammatory myopathies (IIMs) are currently lacking. The purpose of this cross-sectional study is to analyze the relationship among low-density granulocytes (LDGs), neutrophil extracellular traps (NETs), and clinical and immunological features of patients with IIM. Methods: We assessed disease activity, damage accrual, amount of LDGs, NETs, expression of LL-37, and serum cytokines in 65 adult patients with IIM. Differences between groups and correlations were assessed by Kruskal-Wallis, Mann-Whitney U, and Spearman ρ tests. The association between LDGs, NETs, disease activity, calcinosis, and cutaneous ulcers was assessed by logistic regression. To address the capacity of LDGs and NETs to diagnose disease activity, we used receiving operating characteristic curves. Results: Low-density granulocytes were higher in patients with active disease, ulcers, calcinosis, and anti-MDA5 antibodies, which correlated with serum levels of IL-17A and IL-18. Neutrophil extracellular traps were higher in patients with calcinosis, elevated titers of antinuclear antibodies, and positive anti-PM/Scl75 tests. The combination of a high proportion of both total LDGs and NETs was associated with the presence of calcinosis and cutaneous ulcers. LL-37 was higher in NETs originating from LDGs. Normal-density neutrophils were elevated in patients with active dermatomyositis. Conclusions: Low-density granulocytes and NETs containing LL-37 are increased in patients with IIM and active disease, and correlate with proinflammatory cytokines. Both total and CD10+ LDGs are potential biomarkers for disease activity and, in combination with NETs, have the potential to detect patients who are at risk for cutaneous ulcers and calcinosis. Competing Interests: The authors declare no conflict of interest. (Copyright © 2021 Wolters Kluwer Health, Inc. All rights reserved.)
Databáze:	MEDLINE
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