The utility of plasma circulating cell-free messenger RNA as a biomarker of glioma: a pilot study.

Autor: Ita MI; Department of Neurological Surgery, Cork University Hospital, Wilton, Cork, Ireland. 118227173@umail.ucc.ie.; Department of Academic Surgery, University College Cork, Cork, Ireland. 118227173@umail.ucc.ie., Wang JH; Department of Academic Surgery, University College Cork, Cork, Ireland., Toulouse A; Department of Anatomy and Neuroscience, University College Cork, Cork, Ireland., Lim C; Department of Neurological Surgery, Cork University Hospital, Wilton, Cork, Ireland., Fanning N; Department of Neuroradiology, University College Cork, Cork, Ireland., O'Sullivan M; Department of Neurological Surgery, Cork University Hospital, Wilton, Cork, Ireland., Nolan Y; Department of Anatomy and Neuroscience, University College Cork, Cork, Ireland., Kaar GF; Department of Neurological Surgery, Cork University Hospital, Wilton, Cork, Ireland., Redmond HP; Department of Academic Surgery, University College Cork, Cork, Ireland.
Jazyk: angličtina
Zdroj: Acta neurochirurgica [Acta Neurochir (Wien)] 2022 Mar; Vol. 164 (3), pp. 723-735. Date of Electronic Publication: 2021 Oct 13.
DOI: 10.1007/s00701-021-05014-8
Abstrakt: Background: Research into the potential utility of plasma-derived circulating cell-free nucleic acids as non-invasive adjuncts to radiological imaging have been occasioned by the invasive nature of brain tumour biopsy. The objective of this study was to determine whether significant differences exist in the plasma transcriptomic profile of glioma patients relative to differences in their tumour characteristics, and also whether any observed differences were representative of synchronously obtained glioma samples and TCGA glioma-derived RNA.
Methods: Blood samples were collected from twenty glioma patients prior to tumour resection. Plasma ccfmRNAs and glioma-derived RNA were extracted and profiled.
Results: BCL2L1, GZMB, HLA-A, IRF1, MYD88, TLR2, and TP53 genes were significantly over-expressed in glioma patients (p < 0.001, versus control). GZMB and HLA-A genes were significantly over-expressed in high-grade glioma patients (p < 0.001, versus low-grade glioma patients). Moreover, the fold change of the BCL2L1 gene was observed to be higher in patients with high-grade glioma (p = 0.022, versus low-grade glioma patients). There was positive correlation between the magnitude of fold change of differentially expressed genes in plasma- and glioma-derived RNA (Spearman r = 0.6344, n = 14, p = 0.017), and with the mean FPKM in TCGA glioma-derived RNA samples (Spearman r = 0.4614, n = 19, p < 0.05). There was positive correlation between glioma radiographic tumour burden and the magnitude of fold change of the CSF3 gene (r = 0.9813, n = 20, p < 0.001).
Conclusion: We identified significant differential expression of genes involved in cancer inflammation and immunity crosstalk among patients with different glioma grades, and there was positive correlation between their transcriptomic profile in plasma and tumour samples, and with TCGA glioma-derived RNA.
(© 2021. The Author(s).)
Databáze: MEDLINE
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