Biochemical and morphological characterization of freshwater microalga Tetradesmus obliquus (Chlorophyta: Chlorophyceae).

Autor: do Carmo Cesário C; Department of Food Technology, Universidade Federal de Viçosa, Viçosa, Brazil. cristiane.cesario@ufv.br., Soares J; Department of Agricultural Engineering, Universidade Federal de Viçosa, Viçosa, Brazil., Cossolin JFS; Department of General Biology, Universidade Federal de Viçosa, Viçosa, Brazil., Almeida AVM; Department of Plant Physiology, Universidade Federal de Viçosa, Viçosa, Brazil., Bermudez Sierra JJ; Graduate Program in Biotechnology (RENORBIO), Universidade Federal do Ceará, Fortaleza, Brazil., de Oliveira Leite M; Department of Agricultural Engineering, Universidade Federal de Viçosa, Viçosa, Brazil., Nunes MC; Department of Veterinary Medicine, Universidade Federal de Viçosa, Viçosa, Brazil., Serrão JE; Department of General Biology, Universidade Federal de Viçosa, Viçosa, Brazil. jeserrao@ufv.br., Martins MA; Department of Agricultural Engineering, Universidade Federal de Viçosa, Viçosa, Brazil., Dos Reis Coimbra JS; Department of Food Technology, Universidade Federal de Viçosa, Viçosa, Brazil.
Jazyk: angličtina
Zdroj: Protoplasma [Protoplasma] 2022 Jul; Vol. 259 (4), pp. 937-948. Date of Electronic Publication: 2021 Oct 13.
DOI: 10.1007/s00709-021-01712-3
Abstrakt: Tetradesmus is a microalgal genus with biotechnological potential due to its rapid production of biomass, which is plenty in proteins, carbohydrates, lipids, and bioactives. However, its morphology and physiology need to be determined to guide better research to optimize the species cultivation and biocompounds processing. Thus, this study describes the biochemistry and morphology of the strain Tetradesmus obliquus BR003, isolated from a sample of freshwater reservoirs in a Brazilian municipality. In the T. obliquus BR003 dry biomass, we identified 61.6% unsaturated fatty acids, and 3.4% saturated fatty acids. Regarding other compounds, 28.50 ± 1.47 g soluble proteins/100 g, 0.14 ± 0.009 g carotenoids/100 g, 0.76 ± 0.013 g chlorophyll a/100 g, and 0.42 ± 0.015 g chlorophyll b/100 g with a chlorophyll a/b ratio of 1.8 were detected. The main chemical elements found were S, Mg, and P. The cells of BR003 were elliptically curved at the ends and without appendages. Histochemical tests showed carbohydrates distributed in the cytoplasm and pyrenoids, some lipid droplets, and proteins. The cytoplasm is rich in vacuoles, rough endoplasmic reticulum, mitochondria, and chloroplasts. The nucleus has a predominance of decondensed chromatin, and the cell wall has three layers. Chloroplasts have many starch granules and may be associated with a spherical central pyrenoid. To the best of our knowledge, this was the first biochemical description combined with ultrastructural morphological characterization of the strain T. obliquus BR003, grown under standard conditions, to demonstrate specific characteristics of the species.
(© 2021. The Author(s), under exclusive licence to Springer-Verlag GmbH Austria, part of Springer Nature.)
Databáze: MEDLINE
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