| Autor: |
Wong NGK; Department of Chemistry, University of York, Heslington YO10 5DD, UK., Rhodes C; Department of Chemistry, University of York, Heslington YO10 5DD, UK., Dessent CEH; Department of Chemistry, University of York, Heslington YO10 5DD, UK. |
| Jazyk: |
angličtina |
| Zdroj: |
Molecules (Basel, Switzerland) [Molecules] 2021 Oct 03; Vol. 26 (19). Date of Electronic Publication: 2021 Oct 03. |
| DOI: |
10.3390/molecules26196009 |
| Abstrakt: |
The application of electrospray ionisation mass spectrometry (ESI-MS) as a direct method for detecting reactive intermediates is a technique of developing importance in the routine monitoring of solution-phase reaction pathways. Here, we utilise a novel on-line photolysis ESI-MS approach to detect the photoproducts of riboflavin in aqueous solution under mildly alkaline conditions. Riboflavin is a constituent of many food products, so its breakdown processes are of wide interest. Our on-line photolysis setup allows for solution-phase photolysis to occur within a syringe using UVA LEDs, immediately prior to being introduced into the mass spectrometer via ESI. Gas-phase photofragmentation studies via laser-interfaced mass spectrometry of deprotonated riboflavin, [RF - H] - , the dominant solution-phase species under the conditions of our study, are presented alongside the solution-phase photolysis. The results obtained illustrate the extent to which gas-phase photolysis methods can inform our understanding of the corresponding solution-phase photochemistry. We determine that the solution-phase photofragmentation observed for [RF - H] - closely mirrors the gas-phase photochemistry, with the dominant m / z 241 condensed-phase photoproduct also being observed in gas-phase photodissociation. Further gas-phase photoproducts are observed at m / z 255, 212, and 145. The value of exploring both the gas- and solution-phase photochemistry to characterise photochemical reactions is discussed. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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