| Autor: |
Gołąb M; Laboratory for Forensic Chemistry, Department of Analytical Chemistry, Faculty of Chemistry, Jagiellonian University, 30-387 Krakow, Poland., Przybyłowska M; Laboratory for Forensic Chemistry, Department of Analytical Chemistry, Faculty of Chemistry, Jagiellonian University, 30-387 Krakow, Poland., Kubáň P; Department of Bioanalytical Instrumentation, Institute of Analytical Chemistry, Czech Academy of Sciences, 60200 Brno, Czech Republic., Itterheimová P; Department of Bioanalytical Instrumentation, Institute of Analytical Chemistry, Czech Academy of Sciences, 60200 Brno, Czech Republic.; Central European Institute of Technology and Faculty of Science, Masaryk University, 62500 Brno, Czech Republic., Woźniakiewicz M; Laboratory for Forensic Chemistry, Department of Analytical Chemistry, Faculty of Chemistry, Jagiellonian University, 30-387 Krakow, Poland. |
| Abstrakt: |
A fast method for the determination of tropane alkaloids, using a portable CE instrument with a capacitively coupled contactless conductivity detector (CE-C 4 D) was developed and validated for determination of atropine and scopolamine in seeds from Solanaceae family plants. Separation was obtained within 5 min, using an optimized background electrolyte consisting of 0.5 M acetic acid with 0.25% ( w/v ) β-CD. The limit of detection and quantification was 0.5 µg/mL and 1.5 µg/mL, respectively, for both atropine and scopolamine. The developed method was validated with the following parameters-precision (CV): 1.07-2.08%, accuracy of the assay (recovery, RE): 101.0-102.7% and matrix effect (ME): 92.99-94.23%. Moreover, the optimized CE-C 4 D method was applied to the analysis of plant extracts and pharmaceuticals, proving its applicability and accuracy. |
