Intermediate Detection in the Casiopeina-Cysteine Interaction Ending in the Disulfide Bond Formation and Copper Reduction.

Autor: Ramírez-Palma LG; Instituto de Química, Universidad Nacional Autónoma de México, Av. Universidad 3000, C. U., México City 04510, Mexico.; Centro Conjunto de Investigación en Química Sustentable UAEMex-UNAM, Carretera Toluca-Atlacomulco km 14.5, Toluca 50200, Mexico., Espinoza-Guillén A; Facultad de Química, Universidad Nacional Autónoma de México, Av. Universidad 3000, C. U., México City 04510, Mexico., Nieto-Camacho F; Facultad de Química, Universidad Nacional Autónoma de México, Av. Universidad 3000, C. U., México City 04510, Mexico., López-Guerra AE; Facultad de Química, Universidad Nacional Autónoma de México, Av. Universidad 3000, C. U., México City 04510, Mexico., Gómez-Vidales V; Instituto de Química, Universidad Nacional Autónoma de México, Av. Universidad 3000, C. U., México City 04510, Mexico., Cortés-Guzmán F; Instituto de Química, Universidad Nacional Autónoma de México, Av. Universidad 3000, C. U., México City 04510, Mexico.; Centro Conjunto de Investigación en Química Sustentable UAEMex-UNAM, Carretera Toluca-Atlacomulco km 14.5, Toluca 50200, Mexico., Ruiz-Azuara L; Facultad de Química, Universidad Nacional Autónoma de México, Av. Universidad 3000, C. U., México City 04510, Mexico.
Jazyk: angličtina
Zdroj: Molecules (Basel, Switzerland) [Molecules] 2021 Sep 22; Vol. 26 (19). Date of Electronic Publication: 2021 Sep 22.
DOI: 10.3390/molecules26195729
Abstrakt: A strategy to improve the cancer therapies involves agents that cause the depletion of the endogenous antioxidant glutathione (GSH), increasing its efflux out of cells and inducing apoptosis in tumoral cells due to the presence of reactive oxygen species. It has been shown that Casiopeina copper complexes caused a dramatic intracellular GSH drop, forming disulfide bonds and reducing Cu II to Cu I . Herein, through the determination of the [Cu II ]-SH bond before reduction, we present evidence of the adduct between cysteine and one Casiopeina as an intermediate in the cystine formation and as a model to understand the anticancer activity of copper complexes. Evidence of such an intermediate has never been presented before.
Databáze: MEDLINE
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