| Autor: |
Avendaño C; Faculty of Veterinary Sciences, University of Chile, Santiago 8820808, Chile., Vidal S; Faculty of Veterinary Sciences, University of Chile, Santiago 8820808, Chile., Villamizar-Sarmiento MG; Faculty of Veterinary Sciences, University of Chile, Santiago 8820808, Chile.; Department of Sciences and Pharmaceutical Technology, University of Chile, Santiago 8380494, Chile., Guzmán M; Faculty of Veterinary Sciences, University of Chile, Santiago 8820808, Chile.; Nucleus of Applied Research in Veterinary and Agronomic Sciences, NIAVA, Faculty of Veterinary Medicine and Agronomy, Universidad de las Américas, Santiago 9250000, Chile., Hidalgo H; Faculty of Veterinary Sciences, University of Chile, Santiago 8820808, Chile., Lapierre L; Faculty of Veterinary Sciences, University of Chile, Santiago 8820808, Chile., Valenzuela C; Faculty of Veterinary Sciences, University of Chile, Santiago 8820808, Chile., Sáenz L; Faculty of Veterinary Sciences, University of Chile, Santiago 8820808, Chile. |
| Abstrakt: |
The aim of this study was to develop and characterize Salmonella enterica serovar Infantis ( S. Infantis) cochleates protected by encapsulation technology as a potential vaccine and to determine its safety in pullets. Cochleates were encapsulated by two technologies, spray drying and ionotropic gelation at different concentrations (0-15% v/v), and were characterized by physicochemical properties, protein content and Fourier Transform Infrared Spectroscopy (FTIR). The cochleates were white liquid suspensions with tubular shapes and a protein content of 1.0-2.1 mg/mL. After encapsulation by spray drying, microparticles ranged in size from 10.4-16.9 µm, were spherical in shape, and the protein content was 0.7-1.8 mg/g. After encapsulation by ionotropic gelation, beads ranged in size from 1620-1950 µm and were spherical in shape with a protein content of 1.0-2.5 mg/g. FTIR analysis indicated that both encapsulation processes were efficient. The cochleates encapsulated by ionotropic gelation were then tested for safety in pullets. No ill effect on the health of animals was observed upon physical or postmortem examination. In conclusion, this study was the first step in developing a potential oral S. Infantis vaccine safe for poultry using a novel cochleate encapsulation technology. Future studies are needed to determine the effectiveness of the vaccine. |
