Metagenomic analysis provides functional insights into seasonal change of a non-cyanobacterial prokaryotic community in temperate coastal waters.

Autor: Matsumoto K; Department of Genomics and Evolutionary Biology, National Institute of Genetics, Mishima, Shizuoka, Japan., Sakami T; Tohoku National Fisheries Research Institute, Japan Fisheries Research and Education Agency, Shiogama, Miyagi, Japan., Watanabe T; Tohoku National Fisheries Research Institute, Japan Fisheries Research and Education Agency, Shiogama, Miyagi, Japan., Taniuchi Y; Hokkaido National Fisheries Research Institute, Japan Fisheries Research and Education Agency, Kushiro, Hokkaido, Japan., Kuwata A; Tohoku National Fisheries Research Institute, Japan Fisheries Research and Education Agency, Shiogama, Miyagi, Japan., Kakehi S; Tohoku National Fisheries Research Institute, Japan Fisheries Research and Education Agency, Shiogama, Miyagi, Japan., Engkong T; Department of Aquatic Bioscience, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Bunkyo, Tokyo, Japan., Igarashi Y; Department of Aquatic Bioscience, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Bunkyo, Tokyo, Japan., Kinoshita S; Department of Aquatic Bioscience, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Bunkyo, Tokyo, Japan., Asakawa S; Department of Aquatic Bioscience, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Bunkyo, Tokyo, Japan., Hattori M; Graduate School of Advanced Science and Engineering, Waseda University, Shinjuku, Tokyo, Japan., Watabe S; School of Marine Biosciences, Kitasato University, Sagamihara, Kanagawa, Japan., Ishino Y; Department of Bioscience and Biotechnology, Graduate School of Bioresource and Bioenvironmental Sciences, Kyushu University, Fukuoka, Fukuoka, Japan., Kobayashi T; National Research Institute of Fisheries Science, Japan Fisheries Research and Education Agency, Yokohama, Kanagawa, Japan., Gojobori T; Department of Genomics and Evolutionary Biology, National Institute of Genetics, Mishima, Shizuoka, Japan., Ikeo K; Department of Genomics and Evolutionary Biology, National Institute of Genetics, Mishima, Shizuoka, Japan.
Jazyk: angličtina
Zdroj: PloS one [PLoS One] 2021 Oct 12; Vol. 16 (10), pp. e0257862. Date of Electronic Publication: 2021 Oct 12 (Print Publication: 2021).
DOI: 10.1371/journal.pone.0257862
Abstrakt: The taxonomic compositions of marine prokaryotic communities are known to follow seasonal cycles, but functional metagenomic insights into this seasonality is still limited. We analyzed a total of 22 metagenomes collected at 11 time points over a 14-month period from two sites in Sendai Bay, Japan to obtain seasonal snapshots of predicted functional profiles of the non-cyanobacterial prokaryotic community. Along with taxonomic composition, functional gene composition varied seasonally and was related to chlorophyll a concentration, water temperature, and salinity. Spring phytoplankton bloom stimulated increased abundances of putative genes that encode enzymes in amino acid metabolism pathways. Several groups of functional genes, including those related to signal transduction and cellular communication, increased in abundance during the mid- to post-bloom period, which seemed to be associated with a particle-attached lifestyle. Alternatively, genes in carbon metabolism pathways were generally more abundant in the low chlorophyll a period than the bloom period. These results indicate that changes in trophic condition associated with seasonal phytoplankton succession altered the community function of prokaryotes. Our findings on seasonal changes of predicted function provide fundamental information for future research on the mechanisms that shape marine microbial communities.
Competing Interests: The authors have declared that no competing interests exist.
Databáze: MEDLINE
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