Odorant metabolism of the olfactory cleft mucus in idiopathic olfactory impairment patients and healthy volunteers.

Autor: Ijichi C; Technology and Solution Development Center, Institute of Food Science and Technologies, Food Products Division, Ajinomoto Co., Inc., Kawasaki, Japan., Wakabayashi H; Technology and Solution Development Center, Institute of Food Science and Technologies, Food Products Division, Ajinomoto Co., Inc., Kawasaki, Japan.; College of Bioresource Sciences, Nihon University, Kameino, Fujisawa, Japan., Sugiyama S; Technology and Solution Development Center, Institute of Food Science and Technologies, Food Products Division, Ajinomoto Co., Inc., Kawasaki, Japan., Hayashi K; Technology and Solution Development Center, Institute of Food Science and Technologies, Food Products Division, Ajinomoto Co., Inc., Kawasaki, Japan., Ihara Y; Technology and Solution Development Center, Institute of Food Science and Technologies, Food Products Division, Ajinomoto Co., Inc., Kawasaki, Japan., Nishijima H; Department of Otolaryngology-Head and Neck Surgery, The University of Tokyo Graduate School of Medicine, Tokyo, Japan., Touhara K; Department of Applied Biological Chemistry, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Tokyo, Japan., Kondo K; Department of Otolaryngology-Head and Neck Surgery, The University of Tokyo Graduate School of Medicine, Tokyo, Japan.
Jazyk: angličtina
Zdroj: International forum of allergy & rhinology [Int Forum Allergy Rhinol] 2022 Mar; Vol. 12 (3), pp. 293-301. Date of Electronic Publication: 2021 Oct 12.
DOI: 10.1002/alr.22897
Abstrakt: Background: It remains unclear whether the metabolic activity of nasal mucus in the olfactory and respiratory areas is different. Moreover, age- and olfaction-related changes may affect metabolism.
Methods: Hexanal, octanal, and 2-methylbutanal were selected for in vitro metabolism analysis and compared between the olfactory cleft and respiratory mucus of participants < 50-year-old with normal olfaction using gas chromatography mass spectrometry. The metabolic activity of hexanal in the olfactory cleft mucus was further compared between three groups, (1) normal olfaction, age < 50 years old, (2) normal olfaction, age ≥50 years old, and (3) idiopathic olfactory impairment. To characterize the enzyme(s) responsible for aldehyde reduction, we also tested if epalr22897estat and 3,5-dichlorosalicylic acid, types of reductase inhibitors, affect metabolism.
Results: Conversion of aldehydes to their corresponding alcohols was observed in the olfactory cleft and respiratory mucus. The metabolic production of hexanol, octanol, and 2-methybutanol was significantly higher in the olfactory cleft mucus than in the respiratory mucus (p < 0.01). The metabolic conversion of hexanal to hexanol in the mucus of the idiopathic olfactory impairment group was significantly lower than that in the age-matched normal olfaction group. Excluding the nicotinamide adenine dinucleotide phosphate (NADPH) regenerating system from the reaction mixture inhibited metabolism. The addition of either epalr22897estat or 3,5-dichlorosalicylic acid did not inhibit this metabolic conversion.
Conclusions: The enzymatic metabolism of odorants in the olfactory cleft mucus is markedly higher than in the respiratory mucus and decreases in patients with idiopathic olfactory impairment.
(© 2021 ARS-AAOA, LLC.)
Databáze: MEDLINE
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