| Autor: |
Gallego-Lobillo P; Institute of Food Science Research (CIAL), Spanish Council of Scientific Research, (CSIC)-Autonomous University of Madrid (UAM), Campus de la Universidad Autónoma de Madrid, c/Nicolás Cabrera, 9, Madrid E-28049, Spain., Doyagüez EG; Centro de Química Orgánica 'Lora Tamayo' (CSIC), c/Juan de la Cierva, 3, Madrid E-28006, Spain., Jimeno ML; Centro de Química Orgánica 'Lora Tamayo' (CSIC), c/Juan de la Cierva, 3, Madrid E-28006, Spain., Villamiel M; Institute of Food Science Research (CIAL), Spanish Council of Scientific Research, (CSIC)-Autonomous University of Madrid (UAM), Campus de la Universidad Autónoma de Madrid, c/Nicolás Cabrera, 9, Madrid E-28049, Spain., Hernandez-Hernandez O; Institute of Food Science Research (CIAL), Spanish Council of Scientific Research, (CSIC)-Autonomous University of Madrid (UAM), Campus de la Universidad Autónoma de Madrid, c/Nicolás Cabrera, 9, Madrid E-28049, Spain. |
| Abstrakt: |
Trehalose, α-d-glucopyranosyl-(1↔1)-α-d-glucopyranoside, is a disaccharide with multiple effects on the human body. Synthesis of new trehalose derivatives was investigated through transgalactosylation reactions using β-galactosidase from four different species. β-galactosidases from Bacillus circulans ( B. circulans ) and Aspergillus oryzae ( A. oryzae ) were observed to be the best biocatalysts, using lactose as the donor and trehalose as the acceptor. Galactosyl derivatives of trehalose were characterized using nuclear magnetic resonance spectroscopy. Trisaccharides were the most abundant oligosaccharides obtained followed by the tetrasaccharide fraction (19.5% vs 8.2% carbohydrates). Interestingly, the pentasaccharide [β-Gal p -(1→4)] 3 -trehalose was characterized for the first time. Greater oligosaccharide production was observed using β-galactosidase from B. circulans than that obtained from A. oryzae , where the main structures were based on galactose monomers linked by β-(1→6) and β-(1→4) bonds with trehalose in the ending. These results indicate the feasibility of commercially available β-galactosidases for the synthesis of trehalose-derived oligosaccharides, which might have functional properties, excluding the adverse effects of the single trehalose. |
