| Autor: |
MacGregor KA; Physiology, Exercise and Nutrition Research Group, University of Stirling., Rodriguez-Sanchez N; Physiology, Exercise and Nutrition Research Group, University of Stirling., Barwell ND; Department of Diabetes and Endocrinology, Forth Valley Royal Hospital., Gallagher IJ; Physiology, Exercise and Nutrition Research Group, University of Stirling., Moran CN; Physiology, Exercise and Nutrition Research Group, University of Stirling., Di Virgilio TG; Physiology, Exercise and Nutrition Research Group, University of Stirling; td13@stir.ac.uk. |
| Jazyk: |
angličtina |
| Zdroj: |
Journal of visualized experiments : JoVE [J Vis Exp] 2021 Sep 27 (175). Date of Electronic Publication: 2021 Sep 27. |
| DOI: |
10.3791/62635 |
| Abstrakt: |
Studies on adipose tissue are useful in understanding metabolic and other conditions. Human subcutaneous adipose tissue is accessible. With appropriate training and strict adherence to aseptic technique, subcutaneous adipose samples can be safely and efficiently obtained in a non-clinical setting by researchers. Following the administration of local anesthetic lateral to the umbilicus, a 14 G needle attached to a 5 or 10 mL syringe is inserted through the skin into the subcutaneous tissue. Under suction, the syringe is moved in a reciprocating, slicing motion to isolate fragments of adipose tissue. Withdrawing the plunger is enough to ensure that adipose tissue fragments are aspirated through the needle into the syringe. A single biopsy can collect about 200 mg of tissue. This biopsy technique is very safe for both participants and research staff. Following the biopsy, participants can resume most everyday activities, although they should avoid swimming and overly strenuous activities for 48 h to avoid excessive bleeding. Participants can safely undergo 2 biopsies within a single day, meaning that the technique can be applied in before-after acute intervention studies. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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