Non-polar lipid from greenshell mussel (Perna canaliculus) inhibits osteoclast differentiation.

Autor:	Siriarchavatana P; School of Food and Advanced Technology, Massey University, Palmerston North 4442, New Zealand., Kruger MC; School of Health Sciences, Massey University, Palmerston North 4442, New Zealand.; Riddet Centre of Research Excellence, Massey University, Palmerston North 4442, New Zealand., Miller MR; Cawthron Institute, Nelson 7042, New Zealand., Tian H; Sanford Ltd., Auckland 1010, New Zealand., Wolber FM; School of Food and Advanced Technology, Massey University, Palmerston North 4442, New Zealand.; Centre for Metabolic Health Research, Massey University, Palmerston North 4442, New Zealand.
Jazyk:	angličtina
Zdroj:	Bone reports [Bone Rep] 2021 Sep 24; Vol. 15, pp. 101132. Date of Electronic Publication: 2021 Sep 24 (Print Publication: 2021).
DOI:	10.1016/j.bonr.2021.101132
Abstrakt:	The osteoclast-dependent bone resorption process is a crucial part of the bone regulatory system. The excessive function of osteoclasts can cause diseases of bone, joint, and other tissues such as osteoporosis and osteoarthritis. Greenshell mussel oil (GSM), a good source of long chain omega-3 polyunsaturated fatty acids (LCn-3PUFAs), was fractionated into total lipid, polar lipid, and non-polar lipid components and their anti-osteoclastogenic activity tested in RAW 264.7 cell cultures. Osteoclast differentiation process was achieved after 5 days of incubation with RANKL in 24-well culture plates. Introducing the non-polar lipid fraction into the culture caused a lack of cell differentiation, and a reduction in tartrate-resistant acid phosphatase (TRAP) activity and TRAP cell numbers in a dose-dependent manner (50% reduction at the concentration of 20 μg/mL, p < 0.001). Moreover, actin ring formation was significantly diminished by non-polar lipids at 10-20 μg/mL. The bone digestive enzymes released by osteoclasts into the pit formation were also compromised by downregulating gene expression of cathepsin K, carbonic anhydrase II (CA II), matrix metalloproteinase 9 (MMP-9), and nuclear factor of activated T-cells, cytoplasmic 1 (NFATc1). This study revealed that the non-polar lipid fraction of GSM oil contains bioactive substances which possess potent anti-osteoclastogenic activity. Competing Interests: H.S.T. has a conflict of interest as she is employed by Sanford Limited which is a mussel producer and exporter. (© 2021 Published by Elsevier Inc.)
Databáze:	MEDLINE
Externí odkaz:	Zobrazit plný text záznamu