Genetic Background of Antimicrobial Resistance in Multiantimicrobial-Resistant Escherichia coli Isolates from Feces of Healthy Broiler Chickens in Tunisia.

Autor: Abbassi MS; Université de Tunis El Manar, Institut de la Recherche Vétérinaire de Tunisie, 20 Rue Jebel Lakhdhar, Bab Saadoun, Tunis 1006, Tunisia.; Université de Tunis El Manar, Faculté de Médecine de Tunis, Laboratoire de Résistance Aux Antibiotiques LR99ES09, Tunisia., Kilani H; Université de Tunis El Manar, Institut de la Recherche Vétérinaire de Tunisie, 20 Rue Jebel Lakhdhar, Bab Saadoun, Tunis 1006, Tunisia.; Université de Tunis El Manar, Faculté de Médecine de Tunis, Laboratoire de Résistance Aux Antibiotiques LR99ES09, Tunisia., Abid I; Department of Botany and Microbiology, College of Science, King Saud University, Riyadh 11451, Saudi Arabia., Sáenz Y; Área de Microbiología Molecular, Centro de Investigación Biomédica de La Rioja (CIBIR), Logroño, Spain., Hynds P; Environmental Sustainability and Health Institute (ESHI), Technological University Dublin, Grangegorman, Dublin 7, Dublin, Ireland., Lengliz S; Université de Tunis El Manar, Institut de la Recherche Vétérinaire de Tunisie, 20 Rue Jebel Lakhdhar, Bab Saadoun, Tunis 1006, Tunisia., Ben Chehida N; Université de Tunis El Manar, Institut de la Recherche Vétérinaire de Tunisie, 20 Rue Jebel Lakhdhar, Bab Saadoun, Tunis 1006, Tunisia., Boutiba-Ben Boubaker I; Université de Tunis El Manar, Faculté de Médecine de Tunis, Laboratoire de Résistance Aux Antibiotiques LR99ES09, Tunisia.
Jazyk: angličtina
Zdroj: BioMed research international [Biomed Res Int] 2021 Oct 01; Vol. 2021, pp. 1269849. Date of Electronic Publication: 2021 Oct 01 (Print Publication: 2021).
DOI: 10.1155/2021/1269849
Abstrakt: Multiantimicrobial-resistant Escherichia coli isolates are a global human health problem causing increasing morbidity and mortality. Genes encoding antimicrobial resistance are mainly harbored on mobile genetic elements (MGEs) such as transposons and plasmids as well as integrons, which enhance their rapid spread. The aim of this study was to characterize 83 multiantimicrobial-resistant E. coli isolates recovered from healthy broiler chickens. Among 78 tetracycline-resistant isolates, the tetA , tetB , and tetC genes were detected in 59 (75.6%), 14 (17.9%), and one (1.2%) isolates, respectively. The sul1 , sul2 , and sul3 genes were detected 31 (46.2%), 16 (23.8%), and 6 (8.9%) isolates, respectively, among 67 sulfonamide-resistant isolates. The PCR-based replicon typing method showed plasmids in 29 isolates, IncFIB (19), IncI1-I γ (17), IncF (14), IncK (14), IncFIC (10), IncP (8), IncY (3), IncHI2 (1), and IncX (1). The class 1 and 2 integrons were detected in 57 and 2 isolates, respectively; one isolate harbored both integrons. Seven and one gene cassette arrays were identified in class 1 and class 2 integrons, respectively. Our findings show that multiantimicrobial-resistant E. coli isolates from chickens serve as reservoirs of highly diverse and abundant tet and sul genes and plasmid replicons. Such isolates and MGEs pose a potential health threat to the public and animal farming.
Competing Interests: The authors declare that there is no conflict of interest regarding the publication of this paper.
(Copyright © 2021 Mohamed Salah Abbassi et al.)
Databáze: MEDLINE
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