p-Co-BDC/AuNPs-based multiple signal amplification for ultra-sensitive electrochemical determination of miRNAs.

Autor: Ma X; School of Chemistry and Chemical Engineering, Southeast University, Nanjing 211189, PR China., Qian K; School of Chemistry and Chemical Engineering, Southeast University, Nanjing 211189, PR China., Ejeromedoghene O; School of Chemistry and Chemical Engineering, Southeast University, Nanjing 211189, PR China., Kandawa-Schulz M; Department of Chemistry and Biochemistry, University of Namibia, Windhoek, Namibia., Song W; Department of Chemistry and Biochemistry, University of Namibia, Windhoek, Namibia., Wang Y; School of Chemistry and Chemical Engineering, Southeast University, Nanjing 211189, PR China. Electronic address: yihongwang@seu.edu.cn.
Jazyk: angličtina
Zdroj: Analytica chimica acta [Anal Chim Acta] 2021 Oct 23; Vol. 1183, pp. 338979. Date of Electronic Publication: 2021 Aug 21.
DOI: 10.1016/j.aca.2021.338979
Abstrakt: In this work, we report AuNPs-decorated pyrolyzed Co-BDC nanosheets (p-Co-BDC/AuNPs) as high-performance electrocatalyst for developing an electrochemical platform. p-Co-BDC/AuNPs as a new electrocatalyst showed superior electrocatalytic activity towards the electrochemical oxidation of methylene blue (MB). Besides, magnetic p-Co-BDC/AuNPs can be well immobilized on the magnetic glassy carbon electrode without further assistance. The oxidation of MB can be reduced by ascorbic acid. Inspired by this phenomenon, an electrochemical biosensor was constructed based on multiple signal amplification for the diagnosis of miRNAs. Firstly, p-Co-BDC/AuNPs enhanced the electrochemical oxidation of MB. Then, strand displacement amplification reaction can form lots of double helix structure DNA to embed more MB molecules. Finally, ascorbic acid in the electrolyte was utilized to reduce the oxidation of MB and improve the electrochemical signal of MB electro-oxidation. The linear detection range for the detection of miRNAs is 100 aM to 10 nM, and the limit of detection is 86 aM. Furthermore, the constructed biosensor also displayed satisfactory selectivity, good reproducibility, and excellent recovery in the detection of real samples. We are convinced that our proposed multiple signal amplification strategy will provide more promising methods for the diagnosis of cancer.
Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2021 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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