Positive electrostatic therapy of metastatic tumors: selective induction of apoptosis in cancer cells by pure charges.

Autor:	Zandi A; Nanobioelectronic Devices Lab., Cancer Electronics Research Group, School of Electrical and Computer Engineering, Faculty of Engineering, University of Tehran, Tehran, Iran.; Nano Electronic Center of Excellence, Nanoelectronics and Thin Film Lab., School of Electrical and Computer Engineering, Faculty of Engineering, University of Tehran, Tehran, Iran., Rafizadeh-Tafti S; Nanobioelectronic Devices Lab., Cancer Electronics Research Group, School of Electrical and Computer Engineering, Faculty of Engineering, University of Tehran, Tehran, Iran., Shojaeian F; Nanobioelectronic Devices Lab., Cancer Electronics Research Group, School of Electrical and Computer Engineering, Faculty of Engineering, University of Tehran, Tehran, Iran.; School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran., Ali Khayamian M; Nanobioelectronic Devices Lab., Cancer Electronics Research Group, School of Electrical and Computer Engineering, Faculty of Engineering, University of Tehran, Tehran, Iran., Abbasvandi F; ATMP Department, Breast Cancer Research Center, Motamed Cancer Institute, ACECR, Tehran, Iran., Faranoush M; Pediatric Growth and Development Research Center, Institute of Endocrinology and Metabolism, Iran University of Medical Sciences, Tehran, Iran.; Cardio-Oncology Research Center, Rajaie Cardiovascuar Medical & Research Center, Iran University of Medical Sciences, Tehran, Iran., Anbiaee R; Department of Radiation Oncology, Imam Hossein Hospital, Shahid Beheshti University of Medical Sciences, Tehran, Iran., Najafikhoshnoo S; Nanobioelectronic Devices Lab., Cancer Electronics Research Group, School of Electrical and Computer Engineering, Faculty of Engineering, University of Tehran, Tehran, Iran., Hoseinpour P; SEPAS Pathology Laboratory, Tehran, Iran., Assadi S; Nanobioelectronic Devices Lab., Cancer Electronics Research Group, School of Electrical and Computer Engineering, Faculty of Engineering, University of Tehran, Tehran, Iran., Katebi P; Nanobioelectronic Devices Lab., Cancer Electronics Research Group, School of Electrical and Computer Engineering, Faculty of Engineering, University of Tehran, Tehran, Iran., Davari Sh Z; Nanobioelectronic Devices Lab., Cancer Electronics Research Group, School of Electrical and Computer Engineering, Faculty of Engineering, University of Tehran, Tehran, Iran., Shalileh S; Nanobioelectronic Devices Lab., Cancer Electronics Research Group, School of Electrical and Computer Engineering, Faculty of Engineering, University of Tehran, Tehran, Iran., Salemizadeh Parizi M; Nanobioelectronic Devices Lab., Cancer Electronics Research Group, School of Electrical and Computer Engineering, Faculty of Engineering, University of Tehran, Tehran, Iran., Vanaei S; Nanobioelectronic Devices Lab., Cancer Electronics Research Group, School of Electrical and Computer Engineering, Faculty of Engineering, University of Tehran, Tehran, Iran., Ghaderinia M; Nanobioelectronic Devices Lab., Cancer Electronics Research Group, School of Electrical and Computer Engineering, Faculty of Engineering, University of Tehran, Tehran, Iran., Abadijoo H; Nanobioelectronic Devices Lab., Cancer Electronics Research Group, School of Electrical and Computer Engineering, Faculty of Engineering, University of Tehran, Tehran, Iran., Taheri P; Department of Stem Cells and Developmental Biology, Cell Science Research Center, Royan Institute for Stem Cell Biology and Technology, ACECR, Tehran, Iran., Reza Esmailinejad M; Department of Surgery and Radiology, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran., Sanati H; ATMP Department, Breast Cancer Research Center, Motamed Cancer Institute, ACECR, Tehran, Iran., Reza Rostami M; Nanobioelectronic Devices Lab., Cancer Electronics Research Group, School of Electrical and Computer Engineering, Faculty of Engineering, University of Tehran, Tehran, Iran., Sadeghian R; Nanobioelectronic Devices Lab., Cancer Electronics Research Group, School of Electrical and Computer Engineering, Faculty of Engineering, University of Tehran, Tehran, Iran., Kordehlachin Y; Nanobioelectronic Devices Lab., Cancer Electronics Research Group, School of Electrical and Computer Engineering, Faculty of Engineering, University of Tehran, Tehran, Iran., Sadegh Mousavi-Kiasary SM; Nanobioelectronic Devices Lab., Cancer Electronics Research Group, School of Electrical and Computer Engineering, Faculty of Engineering, University of Tehran, Tehran, Iran., Mamdouh A; Nanobioelectronic Devices Lab., Cancer Electronics Research Group, School of Electrical and Computer Engineering, Faculty of Engineering, University of Tehran, Tehran, Iran., Hossein Miraghaie S; Nanobioelectronic Devices Lab., Cancer Electronics Research Group, School of Electrical and Computer Engineering, Faculty of Engineering, University of Tehran, Tehran, Iran., Baharvand H; Department of Stem Cells and Developmental Biology, Cell Science Research Center, Royan Institute for Stem Cell Biology and Technology, ACECR, Tehran, Iran.; Department of Developmental Biology, University of Science and Culture, Tehran, Iran., Abdolahad M; Nanobioelectronic Devices Lab., Cancer Electronics Research Group, School of Electrical and Computer Engineering, Faculty of Engineering, University of Tehran, Tehran, Iran.; Nano Electronic Center of Excellence, Nanoelectronics and Thin Film Lab., School of Electrical and Computer Engineering, Faculty of Engineering, University of Tehran, Tehran, Iran.; Cancer Institute, Imam Khomeini Hospital, Tehran University of Medical Sciences, Tehran, Iran.; UT&TUMS Cancer electronic Research Center, Tehran University of Medical Sciences, Tehran, Iran.
Jazyk:	angličtina
Zdroj:	Cancer medicine [Cancer Med] 2021 Nov; Vol. 10 (21), pp. 7475-7491. Date of Electronic Publication: 2021 Oct 09.
DOI:	10.1002/cam4.4267
Abstrakt:	Background: We discovered that pure positive electrostatic charges (PECs) have an intrinsic suppressive effect on the proliferation and metabolism of invasive cancer cells (cell lines and animal models) without affecting normal tissues. Methods: We interacted normal and cancer cell lines and animal tumors with PECs by connecting a charged patch to cancer cells and animal tumors. many biochemical, molecular and radiological assays were carried out on PEC treated and control samples. Results: Correlative interactions between electrostatic charges and cancer cells contain critical unknown factors that influence cancer diagnosis and treatment. Different types of cell analyses prove PEC-based apoptosis induction in malignant cell lines. Flowcytometry and viability assay depict selective destructive effects of PEC on malignant breast cancer cells. Additionally, strong patterns of pyknotic apoptosis, as well as downregulation of proliferative-associated proteins (Ki67, CD31, and HIF-1α), were observed in histopathological and immunohistochemical patterns of treated mouse malignant tumors, respectively. Quantitative real-time polymerase chain reaction results demonstrate up/down-regulated apoptotic/proliferative transcriptomes (P21, P27, P53/CD34, integrin α5, vascular endothelial growth factor, and vascular endothelial growth factor receptor) in treated animal tumors. Expression of propidium iodide in confocal microscopy images of treated malignant tissues was another indication of the destructive effects of PECs on such cells. Significant tumor size reduction and prognosis improvement were seen in over 95% of treated mouse models with no adverse effects on normal tissues. Conclusion: We discovered that pure positive electrostatic charges (PECs) have an intrinsic suppressive effect on the proliferation and metabolism of invasive cancer cells (cell lines and animal models) without affecting normal tissues. The findings were statistically and observationally significant when compared to radio/chemotherapy-treated mouse models. As a result, this nonionizing radiation may be used as a practical complementary approach with no discernible side effects after passing future human model studies. (© 2021 The Authors. Cancer Medicine published by John Wiley & Sons Ltd.)
Databáze:	MEDLINE
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